|Table of Contents|

Construction Regeneration System of Populus nigra cv.‘Jinye’ Tissue Culture

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年09
Page:
97-103
Research Field:
Publishing date:

Info

Title:
Construction Regeneration System of Populus nigra cv.‘Jinye’ Tissue Culture
Author(s):
ZHANG Heyan; YE Dongmei; LIN Tao; PENG Peng; HUA Jiawen; YU Xiaoqian
(Forestry College,Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010019)
Keywords:
Populus nigra cv. ‘Jinye’; tissue culture; axillary bud induction culture
PACS:
-
DOI:
10.11937/bfyy.20192450
Abstract:
The stem segment with axillary buds of Populus nigra cv.‘Jinye’ were used as explant,the method of tissue culture was adopted,through the selection of the most suitable culture medium,the axillary bud induction,subculture proliferation and rooting were studied,in order to provide the basic research theory for maintaining its excellent characters and realizing the factory seedling.The results showed that the best disinfection method for annual stems was 0.1%HgCl2 for 10 minutes.For axillary bud induction using stem,the highest induction frequency of axillary bud was 92.6%,using MS containing 6-BA 0.5 mg·L-1 and NAA 0.3 mg·L-1.The best hormone ratio for proliferation was 6-BA 1.0 mg·L-1 and NAA 0.4 mg·L-1,additional GA3 0.2 mg·L-1.On this medium,the multiplication coefficient could reach to 4.4,we inducted seedling rooting on 1/2MS and 0.5 mg·L-1 IBA,the rooting rate was 91.2%.

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Last Update: 2020-07-24