|Table of Contents|

Comparative Study on the Main Constituents and Scavenging DPPH Free Radicals of Root，Stem and Leaf of Four Dendrobium Plants

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年10
Page:
128-133
Research Field:
Publishing date:

Info

Title:
Comparative Study on the Main Constituents and Scavenging DPPH Free Radicals of Root，Stem and Leaf of Four Dendrobium Plants
Author(s):
CHEN Jing; CHEN Naidong; ZHENG Quan
(College of Biotechnology and Pharmaceutical Engineering，West Anhui University，Liuan，Anhui 237012)
Keywords:
Dendrobium; extract; polysaccharide; polyphenol; DPPH free radicals
PACS:
-
DOI:
10.11937/bfyy.20192434
Abstract:
The yield of the water and ethanol extracts as well as the polysaccharide content，polyphenol content and the DPPH free radicals scavenging activity from the roots，stems and leaves of Dendrobium huoshanense，Dendrobium officinale，Dendrobium moniliforme and Dendrobium henanense, were analyzed to provide references for the utilization of Dendrobiums，by condensation reflux method，anthracene-sulfuric acid colorimetric method and the colorimetric method of Folin-Ciocalteu and DPPH free radicals method，respectively.The results showed that the yield of water extract and the content of total polysaccharide were stem＞leaf＞root.The yield of alcohol extract of D.huoshanese，D.officinale and D.henanese were leaf＞root＞stem，and D.moniliforme was leaf＞stem＞root.The content of total polyphenol in all the four experimental Dendrobiums were root＞leaf＞stem；both the water and ethanol extracts from the root showed the best DPPH free radicals scavenging ability.From our investigation，it could be concluded that the content of the water extract，ethanol extract，total polysaccharide，the total polyphenol and the DPPH free radicals scavenging activity of the root，stem and leaves are quite different.Using the contents of the total polysaccharide as the quality evaluation standards，the stems of the four Dendrobiums are the most proper to use as the drug.Using the the content of the total polyphenol and the DPPH free radicals scavenging activity as the quality evaluation standards，the root might be the best medicinal parts of D.huoshanese，D.officinale,D.moniliforme and D.henanese.

References:

[1]HAW J W，CHEN N F，CHEN N D，et al.Rapid quantification of polysaccharide and the main monosaccharides in Dendrobium huoshanense by near-infrared attenuated total reflectance spectroscopy[J].Journal of Pharmaceutical and Biomedical Analysis,2018,151:331-338.[3]CHEN N D，YOU T，LI J，et al.A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromate graphyemass spectrometry combined with chemometric methods[J].Journal of Food and Drug Analysis,2016，24(4):839-847.[2]SI H Y，CHEN N F，CHEN N D，et al.Structural characterisation of a water-soluble polysaccharide from tissue-cultured Dendrobium huoshanense C.Z.Tang et S.J.Cheng[J].Natural Product Research，2018,32(3):252-260.[4]CHEN N D，CHEN H，LI J，et al.Discrimination and similarity evaluation of tissue-cultured and wild Dendrobium species using fourier transform infrared spectroscopy[J].Journal of Molecular Structure，2015，1086(4):255-265.[5]CHEN N D，CHEN N F，LI J，et al.Rapid authentication of different ages of tissue-cultured and wild Dendrobium huoshanense as well as wild Dendrobium henanense using FTIR and 2D-COS IR[J].Journal of Molecular Structure，2015,1101:101-108.[6]CHEN N D，CHEN N F，LI J，et al.Similarity evaluation of different origins and species of Dendrobiums by GC-MS and FTIR analysis of polysaccharides[J].International Journal of Analytical Chemistry，2015.[7]魏明，刘艳艳，蔡为荣，等．霍山石斛多酚超声波辅助提取工艺优化及其抗氧化活性研究[J].食品与机械，2016(7):136-140.[8]GIRI L，DHYANI P，RAWAT S，et al.In vitro production of phenolic compounds and antioxidant activity in callus suspension cultures of habenaria edgeworthii:A rare himalayan medicinal orchid[J].Industrial Crops and Products,2012,39:1-6.[9]黄琴，沈杨霞，张成静，等.铁皮石斛多酚和黄酮含量及与抗氧化活性的相关性[J].应与环境生物学报，2014(3):438-442.[10]陈乃东，陈乃富，王陶陶，等.组培霍山石斛、野生霍山石斛及河南石斛多糖及乙醇溶出物动态积累规律研究[J].天然产物研究与开发，2015，27(12):2090-2094.[11]MASSIMO M，LINA C，FEDERICA M，et al.Antigenotoxic effect，composition and antioxidant activity of Dendrobium speciosum[J].Food Chemistry，2013，140(4)：660-665.[12]TRUONG M，DO K，PHUNG T，et al.Phenolic compounds and antioxidant activity of phalaenopsis orchid hybrids[J].Antioxidants,2016,5(3)：31-42.[13]周桂芬，吕圭源.铁皮石斛不同部位黄酮碳苷类成分及清除DPPH自由基能力比较研究[J].中国中药杂志，2012,37(11):1536-1540.

Memo

Memo:
-
Last Update: 2020-07-26