|Table of Contents|

Analysis on Genetic Diversity of Schisandra chinensis Baill.Germplasms Based on NCBI Data

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年05
Page:
109-114
Research Field:
Publishing date:

Info

Title:
Analysis on Genetic Diversity of Schisandra chinensis Baill.Germplasms Based on NCBI Data
Author(s):
WANG He1; YANG Na1; SUN Mingyang1; GUAN Xin1; WANG Changbao1; 2
(1.Jiamusi University,Jiamusi,Heilongjiang 154007;2.Yulin Normal University，Yulin,Guangxi 537000)
Keywords:
Schisandra chinensis Baill; sequence character; genetic diversity; phylogenetic analysis
PACS:
-
DOI:
10.11937/bfyy.20192250
Abstract:
The DNA sequence of Schisandra chinensis Baill in the NCBI nucleic acid database were taken as the research object.The sequence characteristics of the four target fragments of ITS2,PEPC,trnH-psbA and matK were studied by using MEGA 6.0 and DnaSP6 software analysis methods,in order to obtain the genetic diversity and germplasm of Schisandra source of resources.The results showed that fifteen variable sites were found among ITS2 sequences;eleven haplotypes were found among PEPC sequences;the biggest genetic distance was 0.046 occur in ITS2 sequences;PEPC sequences owned highest haplotype diversity 0.985,ITS2 sequences owned highest nucleotide diversity 0.004 93,and all transition/transversion number of four fragments were less than two;and all phylogenetic trees based on four fragments were divided into three clades.

References:

[1]国家药典委员会.中华人民共和国药典(一部)[M].北京:中国医药科技出版社,2015:66.[2]中国科学院植物志编辑委员会.五味子属,中国植物志30(1)卷[M].北京:科学出版社,1996:252.[3]卓丽环,黄普华,金万昌,等.黑龙江省北五味子一新变种[J].植物研究,1994(1):35-36.[4]艾军,王英平,张庆田,等.五味子种质资源果实性状的种内变异研究[J].北方园艺,2011(13):179-182.[5]李先宽,王冰,郑艳超,等.五味子特殊类型白果五味子的分子标记研究[J].中药材,2014,37(4):568-572.[6]李先宽,王冰,郑艳超,等.五味子特殊类型白果五味子的ITS2序列鉴定研究[J].中国中药杂志,2014,39(11):2011-2015.[7]金银萍,王英平,杨一鸣,等.不同种质资源五味子藤茎中总黄酮、总黄酮醇和总酚酸的含量测定[J].时珍国医国药,2018,29(3):697-699.[8]张正海,张悦,赵伟伟,等.北五味子种质资源调查与分析[J].北方园艺,2013(13):185-187.[9]伊旭,刘权钢,李成录,等.长白山区北五味子种质资源的遗传多样性分析[J].延边大学农学学报,2012,34(4):304-308.[10]王有菊,姜静,金顺玉,等.北五味子遗传多样性的ISSR分析[J].林业科技,2007,32(2):57-59.[11]SUN Y,WEN X Y,HUANG H W.Population genetic differentiation of Schisandra chinensis and Schisandra sphenanthera as revealed by ISSR analysis[J].Biochemical Systematics and Ecology,2010,38(3):257-263.[12]张庆田,李昌禹,许培磊,等.五味子果色相关的SRAP标记鉴定[J].生物技术,2015,25(2):147-150.[13]徐梦平.缙云黄芩(Scutellaria tsinyunensis C.Y.Wu et S.Chow)及近缘种基于ITS和cpDNA序列的遗传多样性研究[D].重庆:西南大学,2016.[14]张怀山.基于SSR标记及ITS、cpDNA序列的刺梨遗传多样性分析[D].贵阳:贵州大学,2017.[15]何文,张静,黄智林,等.基于ITS序列对栽培中国樱桃遗传多样性及其群体遗传结构的分析[J].西北植物学报,2014,34(3):463-472.[16]胡昳恒,党萌,张甜,等.秦岭地区核桃自然群体和栽培群体的遗传多样性及其演化关系：基于nrDNA ITS序列分析[J].林业科学,2014,50(12):47-55.[17]SHI Z G,AN W J,JIAO E N,et al.Genetic polymorphism of eighteen Lycium barbarum resources based on nrDNAITS sequence[J].Agricultural Science & Technology,2008,36(3):53-55.[18]吴淑芳,方慧怡.基于NCBI开放接口的检索和下载文献题录信息功能的实现[J].中华医学图书情报杂志,2017,26(3):41-45.[19]BURLAND T G.DNASTAR′s lasergene sequence analysis software[J].Methods in Molecular Biology,2000,132:71-91.[20]LEWIS P O,KUMAR S,TAMURA K,et al.MEGA6:Molecular evolutionary genetics analysis version 6.0[J].Molecular Biology & Evolution,2013,30(12):2725-2729.[21]ROZAS J,FERRER M A,SANCHEZ D B,et al.DnaSP 6:DNA sequence polymorphism analysis of large data sets[J].Molecular Biology and Evolution,2017,34(12):3299-3302.[22]金顺玉.北五味子甲、乙素的遗传变异及基于ISSR标记的遗传变异研究[D].哈尔滨:东北林业大学,2005.[23]赵芹,谢大森,彭庆务,等.不同产地瓠瓜品种ITS序列的遗传多样性分析[J].植物资源与环境学报,2014,23(3):24-35.[24]叶强,张阳,王霞,等.八个北五味子种质资源的比较分析[J].黑龙江农业科学,2015(2):111-112.[25]MINDELL K D P.Substitution bias,weighting of DNA sequence evolution,and the phylogenetic position of fea′s viper[J].Systematic Biology,1993,42(1):18-31.

Memo

Memo:
-
Last Update: 2020-04-29