|Table of Contents|

Study on the Endosymbionts Diversity in Cacopsylla citrisuga (Hemiptera:Psyllidae) Baseded on 16S rDNA Sequence

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年03
Page:
22-28
Research Field:
Publishing date:

Info

Title:
Study on the Endosymbionts Diversity in Cacopsylla citrisuga (Hemiptera:Psyllidae) Baseded on 16S rDNA Sequence
Author(s):
GAO Wa1; WU Fengnian2; WANG Yanjing3; XU Changbao4; CEN Yijing4
(1.Baotou Academy of Agricultural and Animal Husbandry Sciences,Baotou,Inner Mongolia 014013;2.School of Food Engineering and Biotechnology,Hanshan Normal University,Chaozhou,Guangdong 521041;3.College of Agriculture and Food,Zhejiang A&F University,Hangzhou,Zhejiang 311300;4.Citrus Huanglongbing Research Laboratory/Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province,South China Agricultural University,Guangzhou,Guangdong 510642)
Keywords:
Cacopsylla citrisuga; 16S rDNA; RFLP; endosymbiont; diversity; huanglongbing
PACS:
-
DOI:
10.11937/bfyy.20192185
Abstract:
Cacopsylla citrisuga Yang&Li was an insect vector of huanglongbing.This pest damaged the young leaves and flushes of lemon and pummel trees in Hongde Yunnan Province.A recent study indicated that both nymphs and adults of C.citrisuga carried ‘Candidates Liberibacter asiaticus’,the bacterium associated with huanglongbing.In this study,the endosymbiont 16S rDNA library of C.citrisuga collected from lemon and pomelo trees was built based on the RFLP of 16S rDNA gene,and the diversity of the endosymbionts was analyzed.The results showed that there were nine kinds of endosymbiont in this pest species,including secondary endosymbiont of Psylla/secondary endosymbiont of Cacopsylla,uncultured bacterium,Arsenophonus endosymbiont,Pantoea sp.,uncultured Erwinia sp.,Pseudomonas sp.,Rahnella sp.,Enterobacter sp.and Wolbachieae bacterium.According to the RFLP,each of the OUT was just different from others,indicated that species and quantity of the endosymbionts were different in C.citrisuga of different postplant and gender.

References:

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Last Update: 2020-02-26