|Table of Contents|

Study on the Stem in vitro Culture for Rhododendron moulmainense

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年08
Page:
66-73
Research Field:
Publishing date:

Info

Title:
Study on the Stem in vitro Culture for Rhododendron moulmainense
Author(s):
BAI Yuqing1WANG Dingyue1XIE Lijuan2
(1.Administrative Office of Wutong Mountain National Park,Shenzhen,Guangdong 518004;2.School of Applied Chemistry and Biotechnology,Shenzhen Polytechnic,Shenzhen,Guangdong 518055)
Keywords:
Rhododendron moulmainense Hook.f.stemtissue culture
PACS:
-
DOI:
10.11937/bfyy.20192114
Abstract:
The stems of Rhododendron moulmainense Hook.f.were used as explants,the method of aseptic culture was adopted and the disinfection of explant,basic medium,proliferation culture,bud elongation culture,rooting and transplanting of seedling were studied,in order to set up the rapid propagation system of Rhododendron moulmainense Hook.f..The results showed that the best explant sterilization methodswas that 70% alcohol for 10 seconds,2% NaClO for 15 minutes,washing with distilled water for 5-6 times.Adventitious bud proliferation culture medium was WPM+ZT 2.0 mg ?L-1+NAA 0.5 mg?L-1+30 g?L-1 sucrose,pH 5.0,with bud elongation culture medium WPM+ZT 0.5 mg?L-1+GA3 2.0 mg?L-1+30 g?L-1 sucrose,pH 5.0.The rooting medium was WPM+IAA 0.1 mg?L-1+1 g?L-1 sucrose,pH 5.0.The transplanting matrix was perlite∶peat soil=1∶1 with more than 90% transplanting survival rates.

References:

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Last Update: 2020-07-08