|Table of Contents|

Identification and Sequence Analysis of S-RNase Genes From Prunus humilis Bunge

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年24
Page:
28-35
Research Field:
Publishing date:

Info

Title:
Identification and Sequence Analysis of S-RNase Genes From Prunus humilis Bunge
Author(s):
YIN Yarui; WU Junkai; MA Juze; WANG Haijing; LI Xiaoying; ZHANG Libin
(College of Horticulture Science and Technology，Hebei Normal University of Science and Technology，Qinhuangdao，Hebei 066004)
Keywords:
Prunus humilis Bunge; S-RNase gene; cloning; self-incompatibility
PACS:
-
DOI:
10.11937/bfyy.20191520
Abstract:
The S-RNase gene was cloned from four cultivars of Prunus humilis by homologous cloning and its sequence characteristics were analyzed to determin the genotypes of different cultivars.It could provide reference for parents selection in Prunus humilis hybrid breeding.The results showed that five new S-RNase alleles were cloned and identified，encoding 168-172 amino acids with relative molecular weight of 19.87-20.34 kDa and theoretical PI was 9.60-9.73,whose phosphorylation was mainly serine.The main components of their secondary structure were alpha-helix and irregular curl.Homology comparison and structural analysis revealed that all the five S-RNases belonged to RNase T2 family.The deduced amino acid sequence consisted of five conservative regions (C1，C2，C3，RC4 and C5) and one highly variable region (HV)，which had a conservative structure similar to S-RNase of Prunus，Pyrus and Malus.

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Last Update: 2019-12-30