|Table of Contents|

Establishment of Seventy-two Lotus (Nelumbo spp.) CultivarsFingerprints Using SSR Fluorescent Markers

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2020年04
Page:
65-73
Research Field:
Publishing date:

Info

Title:
Establishment of Seventy-two Lotus (Nelumbo spp.) CultivarsFingerprints Using SSR Fluorescent Markers
Author(s):
LI Qingzhu1 ; XU Junxu1; WANG Zhen1; JI Qin2; GU Yun3; YANG Liuyan1
(1.Forestry and Pomology Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201106;2.Shanghai Guyi Garden,Shanghai 201802;3.College of Agricultural,Anshun University,Anshun,Guizhou 561000)
Keywords:
lotus (Nelumbo spp.); fingerprint; genetic similarity; SSR fluorescent labeling
PACS:
-
DOI:
10.11937/bfyy.20191455
Abstract:
Seventy-two lotus varieties were used as materials,the genetic diversity of different varieties was studied by fluorescence labeled SSR and capillary electrophoresis,and the fingerprints were constructed in order to provide basis for the collection and classification of lotus germplasm resources.The results showed that nine SSR fluorescent labeling markers combined with capillary electrophoresis detection method were used to detect the polymorphism of 72 lotus samples.In total,51 polymorphic alleles were detected,with an average of 5.67 alleles per loci.The polymorphism information content PIC was ranged from 0.34 to 0.76，with an average of 0.56.72 lotus cultivars could be fully distinguished by using 6 pairs of SSR primers,including CL04,SSR449,SSR412,CL01,CL16 and CL10.SSR fingerprints of 72 lotus cultivars were established by combining all the amplified bands of each primer,the clustering dendrogram was further performed by using SSR fingerprint data,and the results showed that 72 samples could be grouped into three major groups at the similarity coefficient of 0.535.This work will provide valuable information for the classification and molecular breeding of lotus cultivars in the future.

References:

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Last Update: 2020-03-30