|Table of Contents|

Identification of Pathogeny of Tomato Canker Disease and Screening of Resistance Varieties

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年23
Page:
20-26
Research Field:
Publishing date:

Info

Title:
Identification of Pathogeny of Tomato Canker Disease and Screening of Resistance Varieties
Author(s):
GAO Wa; ZHANG Dongmei; GAO Zhenjiang; LIU Dan; SHANG Chunming; LIU Mingxing
(Baotou Academy of Agricultural and Animal Husbandry Sciences,Baotou,Inner Mongolia 014013)
Keywords:
bacterial canker of tomato; pathogen identification; resistance screening
PACS:
-
DOI:
10.11937/bfyy.20191132
Abstract:
Using tomato ‘CM966’ disease strain as test material,the suspected tomato disease strain collected by Baotou city was identified by PCR method and morphological identification method,and the phylogenetic tree was constructed to determine its taxonomic status;17 tomato lines and cultivars were tested with specific PCR amplification and using Rcm5.1 with the highest contribution rate in the QTL locus associated with tomato canker disease,and field inoculation test was conducted to explore tomato canker resistant species resources.The results showed that the suspected canker disease strains from four different tomato plantations all carried tomato canker.After BLAST comparison,the isolates of 4 pathogens were more than 90% identical to the reported Clavibacter michiganensis subsp.michiganensis (Cmm) in GenBank,including 1 pathogen.The isolate sequence was significantly different from other strains;17 tomato lines and cultivars were tested,and none of the resistance genes at the Rcm5.1 point in the QTL locus was carried.However,field vaccination trials showed that three strains ‘Shandong Holland’,‘Sheng 490’ and ‘Amethyst’ showed high resistance,with disease indices of 2%,4%,and 4%,respectively.‘160’‘CM966’‘T54’ and ‘Provence’ showed high susceptible,and the disease index was greater than 50%.

References:

[1]罗来鑫,赵廷昌,李健强,等.番茄细菌性溃疡病研究进展[J].中国农业科学,2004,37(8):1144-1150.[2]GLEASON M L,GITAITIS R D,RICKER M D.Recent progress in understanding and controlling bacterial canker of tomato in eastern north America[J].Plant Disease,1993,77:1069-1076.[3]KAMASA J.Clavibacter michiganensis subsp.michiganensis[J].Eppo Bulletin,2013,43(1):46-67.[4]吴兴海,邵秀玲,厉艳,等.应用分子生物学方法快速检测番茄溃疡病菌的研究[J].江西农业学报,2006,18(2):5-7.[5]蔡昭雄,廖家榜,罗标,等.桂林市番茄溃疡病的发生及防治[J].中国蔬菜,2007(7):53-54.[6]何亮,陈群,曾忠铭,等.通过特异PCR扩增和16S rDNA序列分析检测动弯杆菌[J].微生物学报,2009,25(2):6-9.[7]于力,朱龙英,万延慧,等.利用多重PCR反应同时筛选番茄Tr-1和Cf-5基因[J].上海农业学报,2005,45(1):27-30.[8]张先练.植物数量性状的遗传与育种[M].呼和浩特:内蒙古大学出版社,1990.[9]FRANCIS D M,KABELKA E.Resistance to bacterial canker in tomato (Lycopersicon hirsutum LA407) and its progeny derived from crossed to L.esculeentum[J].Plant Disease,2001,85:1171-1175.[10]闵现华.番茄溃疡病菌的富集和快速分子生物学检测方法研究[D].兰州:兰州大学,2010.[11]PARK N Y,CHUNG C Y,MCLAREN A J,et al.Polymerase chain reaction for identification of human and porcine spirochaetes recovered from cases of intestinal spirochaetosis[J].FEMS Microbiology Letters,1995,125(2-3):225-229.[12]COAKER G L.Mapping,genetic effects,and epistatic interaction of two bacterial canker resistance QTLs from Lycopersicon hirsutum[J].Theoretical & Applied Genetics，2004,108(6):1047-1055.[13]王蕊,罗来鑫,李健强.不同来源番茄溃疡病菌致病力差异研究[J].植物保护,2010(1):73-77.[14]曹春梅.不同番茄品种对细菌性溃疡病的抗性测定及其抗病机理的初步研究[D].呼和浩特:内蒙古农业大学,2006.[15]高娃,张冬梅,刘丹,等.番茄溃疡病防治药剂的筛选[J].安徽农业科学,2018,46(20):137-140.[16]MANCEAU C.Assessment of genetic diversity among strains of Pseudomonas syringae by PCR-restriction fragment length polymorphism analysis of rRNA operons with special[J].Appl Environ Microbiol,1997,63(2):498-505.

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Last Update: 2019-12-19