[1]FIOR S,LI M,OXELMAN B,et al.Spatiotemporal econstruction of the Aquilegia rapid radiation through next-generation sequencing of rapidly evolving cpDNA regions[J].New Phytologist,2013,198(2):579-592.[2]中国科学院研究所.中国高等植物科属检索表[M].北京:科学出版社,1979:168-173.[3]Royal Botanic Garden Edinburgh (RBGE).Flora Europaea[EB/OL].https://rbg-web2.rbge.org.uk/cgi-bin/nph-readbtree.pl/feout?FAMILY_XREF=&GENUS_XREF=Aquilegia&SPECIES_XREF=&TAXON_NAME_XREF=&RANK=species,2008.[4]United States Department of Agriculture,USDA Plants Profile:Aquilegia[EB/OL]https://plants.usda.gov/core/profile?symbol=AQUIL,2008.[5]廖腾飞,雷家军.尖萼耧斗菜种子萌发特性研究[J].种子,2011,30(1):92-93.[6]刘影.3种植物生长激素类物质对耧斗菜种子发芽的影响[J].江苏林业科技,2016,43(3):17-19.[7]PORCEDDU M,MATTANA E,PRITCHARD H W,et al.Dissecting seed dormancy and germination in Aquilegia barbaricina,through thermal kinetics of embryo growth[J].Plant Biology (Stuttgart),2017,19(6):983-993.[8]李森,袁晓娜,侯非凡,等.华北耧斗菜(Aquilegia yabeana Kitag)的引种驯化及耐旱性评价[J].河北农业大学学报,2015,38(2):48-71.[9]全雪丽,张美淑,刘迪.尖萼耧斗菜花药发育解剖学研究[J].北方园艺,2011(8):159-161.[10]予茜,郭友好,黄双全,等.三种耧斗菜属植物柱头的特征[J].植物分类学报,2005,43(6):513-516.[11]王立军,张友民,谷安根,等.尖萼耧斗菜幼苗初生维管系统的解剖学研究[J].植物研究,1993,13(2):132-135.[12]孙立.耧斗菜族(毛茛科)三种植物的繁育系统和传粉生物学研究[D].西安:陕西师范大学,2014.[13]WHITTALL J B,HODGES S A.Pollinator shifts drive increasingly long nectar spurs in columbine flowers[J].Nature,2007,447(7145):706-709.[14]GRIFFIN S R,MAVRAGANIS K,ECKERT C G.Experimental analysis of protogyny in Aquilegia canadensis (Ranunculaceae)[J].American Journal of Botany,2000,87(9):1246-1256.[15]BRUNET J,ECKERT C G.Effects of floral morphology and display on outcrossing in blue columbine,Aquilegia caerulea (Ranunculaceae)[J].Functional Ecology,1998,12(4):596-606.[16]BRUNET J.Pollinators of the Rocky Mountain columbine:Temporal variation,functional groups and associations with floral traits[J].Annals of Botany,2009,103(9):1567-1578.[17]THAIRU M W,BRUNET J.The role of pollinators in maintaining variation in flower colour in the Rocky Mountain columbine,Aquilegia coerulea[J].Annals of Botany,2015,115:971-979.[18]费砚良.耧斗菜的引种栽培[J].中国园林,1988(2):55-56.[19]TEPFER S S,GREYSON R I,CRAIG W R,et al.In vitro culture of floral buds of Aquilegia[J].American Journal of Botany,1963,50(10):1035-1045.[20]TEPFER S S,KARPOFF A J,GREYSON R I.Effects of growth substances on excised floral buds of Aquilegia[J].American Journal of Botany,1966,53(2):148-157.[21]BILDERBACK D E,KARPOFF A J,TEPFER S S.Development of excised floral buds of aquilegia:The coconut-milk problem[J].American Journal of Botany,1968,55(9):1042-1046.[22]BILDERBACK D E.The Effects of hormones upon the development of excised floral buds of Aquilegia[J].American Journal of Botany,1972,59(5):525-529.[23]KARPOFF A J.Control of in vitro sepal development of excised floral buds of Aquilegia (Ranunculaceae)[J].American Journal of Botany,1974,61(7):778-786.[24]李春玲,蒋钟仁,熊佑清.早春野生花卉组织培养研究初报[J].园艺学报,1992(3):277-278.[25]李群,赵富群,陈丽萍,等.耧斗菜的组织培养[J].植物生理学报,2009,45(1):45-46.[26]王非,姜思佳,李忠才,等.尖萼耧斗菜外植体消毒及愈伤组织诱导的研究[J].安徽农业科学,2010,38(35):19922-19924.[27]KRAMER E M.Aquilegia:A new model for plant development,ecology,and evolution[J].Annual Review of Plant Biology,2009,60(60):261-277.[28]刘莹.耧斗菜的遗传结构研究[D].长春:东北师范大学,2016.[29]高运玲,陈敏,李生伟,等.基于18S rRNA基因序列的毛茛科及近缘植物的分子进化关系研究[J].四川大学学报(自然科学版),2010,47(2):377-382.[30]孙莹莹.中国东北地区耧斗菜属分子系统发育与遗传结构研究[D].长春:东北师范大学,2017.[31]李森,李婷婷,亢秀萍,等.耧斗菜园艺品种与野生华北耧斗菜亲缘关系的SRAP分析[J].北方园艺,2015(2):98-101.[32]BRUNET J,LARSON-RABIN Z,STEWART C M.The distribution of genetic diversity within and among populations of the Rocky Mountain columbine:The impact of gene flow,pollinators,and mating system[J].International Journal of Plant Sciences,2012,173(5):484-494.[33]LI M R,WANG H Y,DING N,et al.Rapid divergence followed by adaptation to contrasting ecological niches of two closely related columbine species Aquilegia japonica and A.oxysepala[J].Genome Biology and Evolution,2019,11(3):910-930.[34]TAYLOR R J.Interspecific hybridization and its evolutionary significance in the genus Aquilegia[J].Brittonia,1967,19(4):374-390.[35]朱蕊蕊,高亦珂,张启翔.耧斗菜属植物杂交育种和性状遗传分析[J].分子植物育种,2019,17(11):3681-3689.[36]WHITTALL J B,VOELCKEL C,KLIEBENSTEIN D J,et al.Convergence,constraint and the role of gene expression during adaptive radiation:Floral anthocyanins in Aquilegia[J].Molecular Ecology,2006,15(14):4645-4657.[37]KRAMER E M,HOLAPPA L,GOULD B,et al.Elaboration of B gene function to include the identity of novel floral organs in the lower eudicot Aquilegia[J].Plant Cell,2007,19(3):750-766.[38]MIN Y,KRAMER E M.The Aquilegia JAGGED homolog promotes proliferation of adaxial cell types in both leaves and stems[J].New Phytologist,2016,216(2):536-548.[39]MIN Y,BUNN J I,KRAMER E M.Homologs of the STYLISH gene family control nectary development in Aquilegia[J].New Phytologist,2019,221(2):1090-1100.[40]张华丽,王涛,崔荣峰,等.耧斗菜HSF分类、表达分析及AyHSF1的亚细胞定位[J].园艺学报,2015,42(8):1533-1541.[41]GARNER J M,ARMITAGE A M.Influence of cooling and photoperiod on growth and flowering of Aquilegia L.cultivars[J].Scientia Horticulturae,1998,75(1):83-90.[42]GIANFAGNA T J,MERRITT R H.Rate and time of GA4/7 treatment affect vegetative growth and flowering in a genetic line of Aquilegia×hybrida Sims[J].Scientia Horticulturae,2000,83(3):275-281.[43]WHITMAN C M,RUNKLE E S.Determining the flowering requirements of two Aquilegia cultivars[J].Hortscience,2012,47(9):1261-1264.[44]杨阳,亢秀萍,张颖.春化条件对耧斗菜抽薹开花的影响[J].山西农业大学学报(自然科学版),2011,31(5):426-429.[45]杨阳,熊远兵,郝晓泳.干旱胁迫对耧斗菜根解剖结构及生理特性的影响[J].北方园艺,2018(17):82-89.[46]郑德承.尖萼耧斗菜个体发育节律及抗旱性研究[D].哈尔滨:东北林业大学,2009.[47]HENRY D.Genomic,proteomic and metabolomic approaches to study drought responses in Aquilegia[D].Clemson:Clemson University,2009.[48]陈菲.低温胁迫对耧斗菜脯氨酸和可溶性蛋白含量的影响[J].北方园艺,2011(5):29-31.[49]陈曦,张婷,刘志洋.低温胁迫对十种宿根花卉电导率的影响[J].北方园艺,2010(10):121-122.[50]杨阳,王红丽,高平.低温胁迫对耧斗菜叶片形态和生理特性的影响[J].江苏农业科学,2018,46(23):151-153.[51]郝丽,任瑞芬,任才,等.干旱及盐胁迫对2种耧斗菜种子萌发的影响[J].中国农学通报,2017,33(27):82-87.[52]GIANFAGNA T J,MERRITT R H,WILLMOTT J D.632 GA4/7 and light level affect flowering and plant height of new cultivars and genetic lines of Aquilegia×hybrida Sims[J].Hortscience,2000,35(3):504-506.[53]朱蕊蕊,杨姗姗,王宇钢,等.H离子注入耧斗菜干种子对萌发率的影响[J].北方园艺,2009(10):88-90.[54]MUSHTAQ S,AGA M A,QAZI P H,et al.Isolation,characterization and HPLC quantification of compounds from Aquilegia fragrans Benth:Their in vitro antibacterial activities against bovine mastitis pathogens[J].Journal of Ethnopharmacology,2016,178:9-12.[55]冯卫生,苏芳谊,郑晓珂,等.华北耧斗菜的化学成分研究[J].中国药学杂志,2011,46(7):496-499.[56]CHEN S B,GAO G Y,LI Y S,et al.Cytotoxic constituents from Aquilegia ecalcarata[J].Planta Medica,2002,68(6):554-556.[57]余雁.尖萼耧斗菜中有效成分提取方法和其生物活性、抗菌模式的相关研究[D].长沙:中南大学,2007.[58]ADAMSKA T,MYNARCZYK W,JODYNIS-LIEBERT J,et al.Hepatoprotective effect of the extract and isocytisoside from Aquilegia vulgaris[J].Phytotherapy Research,2003,17(6):691-696.[59]NOUTSOS C,PERERA A M,NIKOLAU B J,et al.Metabolomic profiling of the nectars of Aquilegia pubescens and A.canadensis[J].PLoS One,2015,10(5):1-13.[60]RADULOVIC〖JX-+0.8mm〗〖KG-*2〗' N,DEKIC M,ZLATKOVIC〖JX-+0.8mm〗〖KG-*2〗' B,et al.A detailed analysis of volatile constituents of Aquilegia pancicii,Degen,a Serbian steno-endemic species[J].Chemical Papers,2007,61(5):405-409.