|Table of Contents|

Ethyl Acetate Extract of Hydrocotyle vulgaris：Effect on Seed Germination and Analyze on Chemical Composition

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年21
Page:
39-47
Research Field:
Publishing date:

Info

Title:
Ethyl Acetate Extract of Hydrocotyle vulgaris：Effect on Seed Germination and Analyze on Chemical Composition
Author(s):
ZHOU Xu; HU Yaping; ZHANG Yanjing; GE Xiaomin; CHEN Shuifei; DING Hui
(Nanjing Institute of Environmental Sciences，Ministry of Environmental Protection，Nanjing，Jiangsu 210042)
Keywords:
Hydrocotyle vulgaris; ethyl acetate extract; seed germination; GC-MS
PACS:
-
DOI:
10.11937/bfyy.20190753
Abstract:
The mature of Hydrocotyle vulgaris and seeds of seven plant species associated with Hydrocotyle vulgaris were chose，the biological indexes included the relative germinating rate，the index of allelopathic and the ratio of root and shoot length were selected to investigate the effects of ethyl acetate extract of Hydrocotyle vulgaris on seed germination.The GC-MS was selected to identify the chemical composition of ethyl acetate extract of Hydrocotyle vulgaris and rhizosphere soil.The results showed that,1) The ethyl acetate extract of Hydrocotyle vulgaris improved the seed germinating rate of Iris pseudacorus and Pharbitis nil，inhibited therate of Bidens tripartite，showed no significant influences on Echinochloa crusgalli var.Zelayensis，Echinochloa cruspavonis，Cyperus michelianus and Eragrostis pilosa.The ethyl acetate extract of rhizosphere soil improved the seed germinating rates of Iris pseudacorusand Pharbitis nil，inhibited the rate of others plants，and showed more significant influences than the ethyl acetate extract of Hydrocotyle vulgaris；2) The ethyl acetate extract of Hydrocotyle vulgaris and rhizosphere soil showed more significant influences on root growth than seedling growth，and the effect was more noticeable in ethyl acetate extract of rhizosphere soil.There existed some kinds of chemicals in ethyl acetate extract of rhizosphere soil that had more prominent influence；3) There existed large differences between the chemical composition of ethyl acetate extract of Hydrocotyle vulgaris and rhizosphere soil.But the 2,6-di-tert-butyl-p-cresol in rhizosphere soil may be the main substance that affected seed germination of companion plants，and it may be sourced from phenolic compounds such as 2,4-di-tert-butylphenol in Hydrocotyle vulgaris.

References:

[1]RICE E L.Allelopathy (2rk edition)[M].New York：Academic Press，1984.[2]阎飞,杨振明,韩丽梅.植物化感作用（Allelopathy）及其作用物的研究方法[J].生态学报,2000，20(4)：692-696.[3]陈圣宾,李振基.外来植物入侵的化感作用机制探讨[J].生态科学,2005，24(1)：69-74.[4]BAIS H P，VEPACHEDU R，GILROY S，et al.Allelopathy and exotic plant invasion，from molecules and genes to species interactions[J].Science，2002,301：1173-1179.[5]LANKAU R A，ROGERS W E，SIEMANN E.Constraints on the utilization of the invasive Chinese tallow tree Sapium sebiferum by generalist native herbivores in coastal prairies[J].Ecological Entomology，2004，17(8)：386-391.[6]CALLAWAT R M，ASCHEHOUG E T.Invasive plants versus their new and old neighbors：A mechanism for exotic invasion[J].Science，2000，290：521-523.[7]孙庆花,张超，刘国斌,等.黄土丘陵区草本群落演替中先锋种群茵陈蒿浸提液的化感作用[J].生态学报，2016，36(8)：2233-2242.[8]郭晓燕,温婷,张露,等.毛红椿落叶腐解物的化感作用及成分[J].林业科学，2018，54(6)：26-32.[9]BONANOMI G，SIEUREZZA M G，CAPORASO S，et al.Phytotoxicity dynamics of decaying plant materials[J].New Phytologist，2006，169(3)：571-578.[10]FERNANDEZ C，VOIRIOT S，MEVY J P，et al.Regeneration failure of Pinus halepensis mill：The role of autotoxicity and some abiotic environmental parameters[J].Forest Ecology & Management，2008，255(7)：2928-2936.[11]李晓娟,王强,倪慧,等.栗和美国板栗化感作用的比较[J].植物生态学报，2013，37(2)：173-182.[12]陶月红.脂溶性化感物质生物监测方法研究及黄檗果皮化感物质的初步研究[D].北京:中国协和医科大学，2008.[13]DONG M.Morphological responses to local light conditions in clonal deposition and the biodiversity of boreal forest：Implications for the nitrogen critical load[J].Oecologia，1995，101(3)：282-288.[14]缪丽华,季梦成,王莹莹,等.湿地外来植物香菇草(Hydrocotyle vulgaris)入侵风险研究[J].浙江农林大学学报(农业与生命科学版)，2011,30(3)：354-358.[15]LIU R H，CHEN Q W，DONG B C，et al.Effects of vegetative propagule pressure on the establishment of an introduced clonal plant，Hydrocotyle vulgaris[J].Scientific Reports，2014，4(1)：5507-5562.[16]DONG B C，WANG J Z，LIU R H，et al.Soil heterogeneity affects ramet placement of Hydrocotyle vulgaris[J].Plant Ecology，2015，8(1)：91-100.[17]DONG B C，WANG J Z，LIU R H，et al.Effects of heterogeneous competitor distribution and ramet aggregation on the growth and size structure of a clonal plant[J].PLoS One，2013，8(7)：e68557. [18]杨琴琴，缪丽华，洪春桃，等.香菇草水浸提液对3种植物种子萌发和幼苗生长的化感效应[J].浙江农林大学学报，2013，30(3)：354-358.[19]桂富荣，蒋智林，金吉斌，等.紫茎泽兰化感作用对9种草本植物种子萌发的影响[J].生物安全学报，2011，20(4)：331-336.[20]曾任森.化感作用研究中的生物测定方法综述[J].应用生态学报，1999，10(1)：123-126.[21]张自常,谷涛,李永丰，等.不同氮水平下不同稗草对水稻产量形成的影响[J].2016，27(11)：3559-3568.[22]王连平，王汉荣，茹水江，等.芦笋田杂草及地膜覆盖除草作用研究[J].江西农业学报，2006，18(4):126-128.[23]郑丽敏.安阳地区夏玉米田杂草发生规律与防治技术研究[D].郑州:河南农业大学，2009.[24]任颖，何萍，徐杰,等.滦河流域河岸带入侵植物分布特征及其与环境的关系[J].应用生态学报，2017，28(6):1843-1850.[25]FLORY S L，BAUER J T.Experimental evidence for indirect facilitation among invasive plants[J].Journal of Ecology，2014，102：12-18.[26]TURK M A，TAWAHA A M.Allelopathic effect of black mustard (Brassica nigra L.) on germination and growth of wild oat (Avena fatua L.)[J].Crop Protection，2003，22(4)：673-677.[27]NISHIDA N，TAMOSTSU S，NAGATA N，et al.Allelopathic effects of volatile monoterpenoids produced by Salvia leucophylla：Inhibition of cee proliferation and DNA synthesis in the root apical meristem of Brassica campestris seedling[J].Journal of Chemical Ecology，2005，31(5)：1187-1203.[28]胡婉君,马丹炜,王亚男,等.土荆芥挥发油对蚕豆根尖细胞的化感潜力[J].生态学报，2011，31(13)：3694-3690.[29]CRUZ-ORTEGA R，ANAYA A L，HERNANDEZ-BAUTISTA B E，et al.Effects of allelochemical stress produced by Sicyos deppei on seedling root ultrastructure of Phaseolus valgaris and Cucurbita ficifolia[J].Journal of Chemical Ecology，1998，24(12)：2039-2057.[30]MILLER G，SUZUKI N，CIFTCI-YILMAZ S，et al.Reactive oxygen species homeostasis and signalling during drought and salinity stresses[J].Plant，Cell & Environment，2010，33(4)：453-467.[31]CHEN C M，LETNIK I，HACHAM Y，et al.Ascorbate peroxidase 6 protects Arabidopsis desiccating and germinating seeds from stress and mediates cross talk between reactive oxygen species，abscisic acid and auxin[J].Plant Physiology，2014，166(1)：370-383.[32]KATO-NOGUCHI H，MACIA L R，ROGERS W E，et al.Action mechanism of 6-methoxy-2-benzoxazolinone on the inhibition of plant germination[C]//New South Wales，Australia：World Congress on Allelopathy，‘Establishing the Scientific Base’,2005.[33]POPOVICI J，BERTRAND C，JACQUEMOUD D，et al.An allelochemical from Myrica gale with strong phytotoxic against highly invasive Fallopia×bohemica Taxa[J].Molecules，2011，16(3)：2323-2333.[34]CHAVASIRI W，PRUKCHAREON W，SAWASDEE P，et al.Allelochemicals from Hydrocotyle umbellata Linn.[C]//World Congress on Allelopathy，‘establishing the Scientific Base’，Wagga Wagga，New South Wales，Australia，2005，21-26 August.[35]舒骏，成向荣，虞木奎，等.刈割对五节芒化感作用及次生代谢物质的影响[J].草地学报，2016，24(1):76-83.[36]张胜花.两种眼子菜科沉水植物与浮游藻类之间的化感作用研究[D].武汉：中国科学院水生生物研究所，2009.[37]李彩凤，陈明，马凤鸣，等.甜菜根7系分泌物对大豆化感作用研究[J].东北农业大学学报，2016，47(8):21-30.[38]邬彩霞，刘苏娇，赵国琦.黄花草木樨水浸提液中潜在化感物质的分离、鉴定[J].草业学报，2014，23(5):184-192.

Memo

Memo:
-
Last Update: 2019-12-06