|Table of Contents|

Identification of Nematicidal Actinomycete NZ-5 and Optimization of Ferment Conditions

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年13
Page:
59-67
Research Field:
Publishing date:

Info

Title:
Identification of Nematicidal Actinomycete NZ-5 and Optimization of Ferment Conditions
Author(s):
MA Zhe
(Beijing Vocation College of Agriculture,Beijing 102442)
Keywords:
root-knot nematodes; actinomycetes; nematocidal activity; identification; fermentation
PACS:
-
DOI:
10.11937/bfyy.20190355
Abstract:
The root-knot nematodes（J2） was used as test materials.An experiment of nematocidal activities was tested using strain NZ-5,which was a kind of antagonistic actinomycete isolated from soil samples.The results showed that the strain NZ-5 had strong activities against root-knot nematodes（J2）.Culture filtrate of NZ-5 could knock-down 84.36% of the J2 within 24 hours.In order to further apply the high antagonistic strain in field production practice,strain NZ-5 was identified as Streptomyces albidoflavu based on the morphological characteristics,cultural characteristics,physiological characteristics and 16S rDNA gene sequence similarity analysis.Fermentation conditions were optimized.The optimal fermentation parameters were as follow:pH 7,4 days fermentation,210 r?min-1,25 ℃,75 mL medium volume in 250 mL flask，oatmeal and yeast powder as carbon and nitrogen source,respectively.The toxic activity of root-knot nematode (J2) was 93.29%.The optimized fermentation products showed 8.93 percentage point higher toxicity against root-knot nematodes(J2) compared with pre-optimum.Strain NZ-5 could effectively kill root-knot nematodes（J2）,and could be used as an excellent strain for further research and development of nematocidal biological pesticides.

References:

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Last Update: 2019-08-01