|Table of Contents|

Establishment of Molecular Marker for Sm Gene Resistant to Tomato Gray Leaf Spot(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年22
Page:
22-28
Research Field:
Publishing date:

Info

Title:
Establishment of Molecular Marker for Sm Gene Resistant to Tomato Gray Leaf Spot
Author(s):
GAO Cun; SONG Jianjun; DU Chao
(College of Bioscience and Bioengineering,Hebei University of Science and Technology,Shijiazhuang,Hebei 050018)
Keywords:
tomato; gray leaf spot disease; Sm gene; molecular marker
PACS:
-
DOI:
10.11937/bfyy.20190318
Abstract:
Resistant homozygous (Sm/Sm)，resistant heterozygotes（Sm/sm）and susceptible homozygous (sm/sm) were used as experimental materials.Based on the published RFLP markers tightly linked to Sm,specific primers were designed for PCR amplification.Sequence analysis of amplified products was studied to find enzyme cleavage sites.A new CAPS marker was greated closely linked to the tomato gray leaf spot disease resistance gene Sm,in order to provide technical support for tomato breeding in the future.The results showed that T050 marker obtained was a stable and reliable codominant molecular marker,which could be used in marker assistant selection for tomato disease resistance breeding.

References:

[1]李戊清.我国灰叶斑病研究进展[J].中国植保导刊,2015(3):24-27.[2]BRUNO E C,LEONARDO S B,AILTON R,et al.Identification of Solanum (S.lycopersicon) accessions with resistance to Stemphylium solani and S.lycopersici[J].Horticultura Brasileira,2010,28:178-184.[3]刘安敏,孙家栋,陶秀珍，等.保护地番茄灰叶斑病的发生与综合防治[J].中国植保导刊,2004(4):23-24.[4]杨琦凤,潘光辉.重庆地区番茄灰叶斑病的发生与防治[J].南方农业,2012(6):28-29.[5]HENDRIX J W,FRAZIER W A.Studies of the inheritance of Stemphylium resistance intomatoes[J].Hawaii Agric Exp Stn Tech Bull,1949,8:4-23.[6]BEHARE J,LATERROT H,SARFATTI M,et al.Restriction fragment length polymorphism mapping of the Stemphylium resistance gene in tomato[J].Molecular Plant-Microbe Interactions,1991,4(5):489.[7]HAMID A,MATTHEW K,MAJID R,et al.A new genetic linkage map of tomato based on a Solanum lycopersicum × S.pimpinellifolium RIL population displaying locations of candidate pathogen response genes[J].Genom,2009,52(11):935-956.[8]JI Y,SCOTT J W.A CAPS marker linked to the tomato gray leafspot(Stemphyllium sp.) resistance gene Sm[J].Rep Tomato Genet Coop,2009,59:29-31.[9]高建昌,苏晓梅,杜永臣,等.与番茄抗灰叶斑病基因Sm连锁的Indel标记及其扩增引物与应用:201510855183[P].2017-06-09.[10]尹贤贵,王小佳.张赞,等.DNA分子标记及其在番茄遗传育种中的应用[J].西南农业大学学报(自然科学版),2004,26(6):465-470.[11]国艳梅,杜永臣,王孝宣,等.番茄黄化卷叶病毒病(TYLCV)的研究进展[J].中国农业科技导报,2009,11(5):30-35.[12]吴媛媛,李海涛,张子君,等.番茄抗病基因分子标记研究进展[J].贵州农业科学,2010,38(2):27-31.[13]赵丽萍,赵统敏,余文贵,等番茄灰叶斑病研究进展[J].江苏农业学报,2014,30(6):1524-1530.[14]郝炯,渠云芳.DNA分子标记在作物育种中的应用[J],山西农业科学,2009,37(3):81-85.[15]王军卫,张改生,刘宏伟,等.分子标记在作物遗传育种中的应用进展[J].陕西农业科学,2001(1):1-5.[16]LAMMERTS B,BACKES G,VRIEND H.The role of molecular markers and marker assisted selection in breeding for organic agriculture[J].Euphytica,2010,175:51-64.[17]张楠,谢放.高质量加工番茄基因组DNA提取方法的改进[J].湖南农业科学,2011(15):16-20.[18]许艺珊,何晶晶，沈婉娟,等.TA克隆技术的原理与应用[J].技术与应用,2015(6):89-90.[19]殷宪伦,王春涛,孔洋翔,等.利用TA克隆的方法简便构建入门克隆[J].植物分类与资源学报,2012,34(4):397-402.

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Last Update: 2019-12-11