|Table of Contents|

Pathogen Identification and Screening of the Fungicides of Muskmelon Fruit Rot Caused by Penicillium oxalicum

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年18
Page:
52-57
Research Field:
Publishing date:

Info

Title:
Pathogen Identification and Screening of the Fungicides of Muskmelon Fruit Rot Caused by Penicillium oxalicum
Author(s):
SUN Lei1BAI Xue2BAI Qingrong1ZHAO Tingchang2
(1.Institute of Plant Protection,Jilin Agricultural University,Changchun,Jilin 130118;2.Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193)
Keywords:
muskmelonPenicillium oxalicumfruit rotpathogen identificationscreening of the fungicides
PACS:
-
DOI:
10.11937/bfyy.20190305
Abstract:
Pathogens which caused muskmelon fruit rot were gotten by tissue isolation method.Their pathogenicity were confirmed according to the Koch′s Postulation,and all of them were the pathogens.The pathogens were identified as Penicillim oxalicum based on morphological characteristics and rDNA-ITS,NS,NL,CAM and RPB2 sequence analysis.The sensitivity of the representative strain TGQM01 pathogens to 20 fungicides was tested to determine effective treatments for controling muskmelon fruit rot by spore germination method.The results showed that the spores of P.oxalicum was more sensitive to 25% pyraclostrobin EC,17% yraclostrobin?epoxiconazole SC,80% captan WG,70% polyram WG,22.5% picoxystrobin SC,75% trifloxystrobin?tebuconazole WG and 60% pyraclostrobin?metiram WG (0.001 7 mg?L-1EC501.000 0 mg?L-1).Followed by 75% chlorothalonil WP,450 g?L-1 prochloraz EW,500 g?L-1 thiophanatemethyl,25% azoxystrobin SC,250 g?L-1 difenoconazole EC and 12% fluxapyroxad?epoxiconazole EC (1.379 5 mg?L-1EC508.131 5 mg?L-1).These results provided theoretical basis for controlling of the disease.

References:

[1]马跃.透过国际分析,看中国西瓜甜瓜的现状与未来[J].中国瓜菜,2011,24(2):64-67.[2]杨艳涛,张琳,吴敬学.2011年我国西甜瓜市场及产业发展趋势与对策分析[J].北方园艺,2012(15):183-187.[3]李华,杜金歧,殷洛明,等.大棚西甜瓜病害发生特点及防治技术[J].上海农业科技,2003(4):80-81.[4]张丽凤,石佩君,孙静,等.甜瓜炭疽病的发生与防治[J].北方园艺,2010(20):161.[5]张艳苓,卜崇兴,匡开源.甜瓜蔓枯病病原菌的分离培养[J].中国瓜菜,2006(1):31-32.[6]卫月勇.甜瓜细菌性角斑病的发生与防治[J].上海蔬菜,2009(2):85-86.[7]倪秀红,赵杰.防治甜瓜灰霉病的药剂筛选试验研究[J].上海农业科技,2009(1):105-107.[8]方中达.植病研究方法[M].3版.北京:中国农业出版社,1998.[9]王梦奇,刘丽玲,李海洋,等.防风枯斑病的病原学及药剂敏感性[J].吉林农业大学学报,2018,40(1):34-42.[10]吴发红,黄东益,黄小龙,等.几种真菌DNA提取方法的比较[J].中国农学通报,2009,25(80):62-64.[11]LONG B,YE B,LIU Q,et al.Characterization of Penicillium oxalicum SL2 isolated from indoor air and its application to the removal of hexavalent chromium[J].PLoS One,2018,13(1):e0191484.[12]VISAGIE C M,HOUBRAKEN J,FRISVAD J C,et al.Identification and nomenclature of the genus Penicillium[J].Studies in Mycology,2014,78:343-371.[13]刘影,马海霞,杨信东.用EXCEL中的VBA编写植物病害流行学常用程序软件包[J].吉林农业大学学报,2008,30(4):436-441.[14]孔华忠.中国真菌志.青霉属及其相关有性型属[M].35卷.北京:科学出版社,2007.[15]VU D,GROENEWALD M,VRIES M D,et al.Large-scale generation and analysis of filamentous fungal DNA barcodes boosts coverage for kingdom Fungi and reveals thresholds for fungal species and higher taxon delimitation[J].Studies in Mycology,2019,92(1):135-154.[16]GUEVARA-SUAREZM,SUTTON D A,CANO-LIRA J F,et al.Penicillium-like fungi from clinical samples in the USA and their antifungal susceptibility[J].Journal of Clinical Microbiology,2016,54(8):2155-2161.[17]VISAGIE C M HOUBRAKEN J,DIJKSTERHUIS J,et al.A taxonomic review of Penicillium species producing conidiophores with solitary phialides,classified in section Torulomyces[J].Persoonia Molecular Phylogeny and Evolution of Fungi,2016,36(1):134-155.[18]HOUBRAKEN J,SAMSON R A.Phylogeny of Penicillium and the segregation of Trichocomaceae into three families[J].Studies in Mycology,2011(70):1-51.[19]张翠香,李娜,李倩,等.柑橘青霉菌的分离鉴定与特性分析[J].华中师范大学学报(自然科学版),2014,48(1):86-90.[20]郭晓敏.桃、李果实采后病害发生特点及其控制措施研究[D].北京:北京工商大学,2011.[21]程小梅,彭亚军,杨玉,等.猕猴桃青霉病病原菌鉴定及中草药提取物对其抑菌效果[J].植物保护,2018,44(3):186-189.

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Last Update: 2019-09-29