|Table of Contents|

TAN Wei1,2,ZHAO Qifeng2,LI Qingliang3

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年17
Page:
41-49
Research Field:
Publishing date:

Info

Title:
TAN Wei1,2,ZHAO Qifeng2,LI Qingliang3
Author(s):
TAN Wei1; 2; ZHAO Qifeng2; LI Qingliang3
(1.College of Food Science and Pharmaceutical Engineering,Zaozhuang University,Zaozhuang,Shandong 277160；2.Pomology Institute,Shanxi Academy of Agricultural Sciences,Shanxi Key Laboratory of Germplasm Improvement and Utilization in Pomology,Taigu,Shanxi 030815；3.College of Life Sciences,Zaozhuang University,Zaozhuang,Shandong 277160)
Keywords:
winegrape; SSR markers; genetic diversity
PACS:
-
DOI:
10.11937/bfyy.20190175
Abstract:
In order to effectively distinguish and rationally use wine grape germplasm resources,and reduce the blindness of parental selection in winegrape breeding,the genetic diversity of twenty-nine winegrape varieties was analyzed by SSR molecular marker technology.The results showed that 10 pairs of SSR primers amplified 90 loci,the observed heterozygosity,the expected heterozygosity and the Shannon diversity index was 0.482 8-0.965 5,0.592 3-0.893 5,1.185 7-2.227 9,and the percentage of polymorphic loci was 100%.The results of UPGMA cluster analysis showed that the 29 materials were divided into two groups at the genetic similarity coefficient of 0.16.Among the Group I including twenty-eight varieties,‘Gamay Teinturier’ and ‘Saperavi’ were classified into the subgroup II；in the Subgroup I,six Vitis vinifera species (‘Merlot 181’‘Cabernet Franc 327’‘Cabernet Sauvignon 169’‘Cabernet Gernischt’‘Marselan c980’‘Ruby Cabernet’) had parent-child relationship,so they classified into one category (Season Group II).The season Group I could classified into Group A and Group B；the former one contains fourteen Vitis vinifera species,one Vitis quinquangularis Rehd.×Vitis vinifera hybrid,one interspecific hybrid,one Vitis amurensis Rupr.×Vitis vinifera hybrid,while the latter one includes one interspecific hybrid and three hybrids between Vitis vinifera and Vitis amurensis Rupr..The UPGMA dendrogram were basically consistent with the pedigree.The results provide effective information for the protection of wine grape germplasm resources and provide theoretical guidance for the selection of parents for wine grape breeding.

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Last Update: 2019-09-24