|Table of Contents|

Screening,Identification and Control of Antagonistic Bacterialon Leaf Blight of Lycium barbarum

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年15
Page:
23-29
Research Field:
Publishing date:

Info

Title:
Screening,Identification and Control of Antagonistic Bacterialon Leaf Blight of Lycium barbarum
Author(s):
LIU Wei1; HE Cai1; JIN Na1; LI Dong1; SHI Xingyun1; LIU Peng2
(1.Wuwei Academy of Forestry Science,Wuwei,Gansu 733000;2.Biomass and Bioenergy Research Centre,College of Plant Science & Technology,Huazhong Agricultural University,Wuhan,Hubei 430070)
Keywords:
leaf blight of Lycium barbarum; antagonistic bacteria; antifungal activity; control effect
PACS:
-
DOI:
10.11937/bfyy.20190155
Abstract:
In order to obtain bacillus which has strong antagonistic effect on leaf blight of Lycium barbarum,151 strains of bacillus were isolated and purified from farmland soil,and found a strain LKYLW-1 that antibacterial bandwidth was 15.2 mm with good antagonistic activity against leaf blight of L.barbarum.The antibacterial effect of LKYLW-1 was measured by pair culture,spore germination and the field efficacy trials.The results showed that the strain LKYLW-1 had teratogenic effects on the mycelium of the leaf blight of L.barbarum.The fermentation broth of strain LKYLW-1 could inhibit the spore germination,and the EC50 was 9.35 mL?L-1.In the field efficacy trials,the antagonistic strain LKYLW-1 showed good preventive and therapeutic effects on leaf blight of L.barbarum.The strain LKYLW-1 was identified as Bacillus atrophaeus by morphological characteristics,physical and chemical analysis and 16S rDNA sequence analysis,which could produce cellulase,protease and amylase during metabolism.So the strain LKYLW-1 has potential utilization value on the control of leaf blight of L.barbarum.

References:

[1]薛福祥.枸杞主要病虫害的识别与防治[J].甘肃科技纵横,2009,38(3):52-53.[2]姜海燕.枸杞叶枯病病原菌研究[D].内蒙古:内蒙古农业大学,2003.[3]刘伟,张勤德,王多文,等.枸杞新病害叶枯病症状表现及病原菌鉴定[J].北方园艺,2018(12):141-145.[4]沙月霞,王琦,李燕.稻瘟病生防芽胞杆菌的筛选及防治效果[J].中国生物防治学报，2016,32(4):474-484.[5]郭芳芳,谢镇,卢鹏.一株多粘类芽孢杆菌的鉴定及其生防促生效果初步测定[J].中国生物防治学报,2014,30(4):489-496.[6]LUGTENBERG B,KAMILOVA F.Plant-growth-promoting rhizobacteria[J].Annual Review of Microbiology,2009,63:541-556.[7]NIEMANN H,LOSEKANN T,de BEER D,et al.Novel microbial communities of the Haakon Mosby mud volcano and their role as a methane sink[J].Nature,2006,443(5227):854-858.[8]周棱波,黎定军,陈武,等.烟草赤星病拮抗菌的筛选[J].湖南农业科学,2011(3):100-101,104.[9]储慧清,方敦煌,孔光辉,等.拮抗菌AM6代谢产物防治烟草赤星病试验[J].烟草科技,2004(4):42-44.[10]李爱荣,安德荣.两株生防荧光假单胞杆菌的室内筛选试验[J].微生物学杂志,2003,23(4)11-13.[11]慕立义,吴文君,王开运.植物化学保护研究方法[M].北京:中国农业出版社,1997.[12]吴文君.植物化学保护实验技术指导[M].西安:陕西科学与技术出版社,1998.[13]康兴娇,申红妙,贾招闪,等.葡萄霜霉病生防菌甲基营养型芽胞杆菌T3的鉴定及其防治效果[J].中国生物防治学报,2016,32(6):775-782.[14]柳凤,欧雄常,何红,等.红树内生菌AmS2菌株对芒果炭疽病菌的抑制作用[J].植物保护学报,2010,37(5):453-458.[15]TODOROV S D,MEINCKEN M,DICKS L M T.Factors affecting the adsorption of bactcriocins ST194BZ and ST23LD to Lactobacillus sakei and Enterococcns sp.[J].Journal of General and Applied Microbiology,2006,52:159-167.[17]刘永刚,李佳佳,李昭煜,等.枸杞炭疽病防治药剂筛选[J].植物保护,2016,42(1):230-234.[18]乔俊卿,陈志谊,梁雪杰.等.枯草芽孢杆菌Bs916防治番茄青枯病[J].中国生物防治学报,2016,32(2):229-234.[19]鹿秀云,马平,李社增,等.一种用于防治棉铃疫病的萎缩芽孢杆菌及其微生物菌剂:中国,CN201210028087.4[P].2012-10-24.[20]辛海峰,孟艳艳,李建宏,等.一株萎缩芽孢杆菌在小麦中的定植及对赤霉病的防治[J].生态学杂志,2013,32(6):1490-1496.[21]韩松,张守村,黄晓艳,等.一株产铁载体内生细菌对尖孢镰刀菌的拮抗作用[J].西北植物学报,2011,31(5):1039-1044.[22]魏海雷,王烨,张力群,等.生防菌株2P24与CPF-10的鉴定及其生防相关性状的初步分析[J].植物病理学报,2004,34(1):80-85.[23]陈丽华,张爱香,朱韬,等.禾谷丝核菌拮抗细菌的鉴定及其拮抗产物分析[J].植物病理学报,2008,38(1):88-95.[24]ARORA N K,KIM M J,KANG S C,et al.Role of chitinase and β-1,3-glucanase activities produced by a fluorescent pseudomonad and in vitro inhibition of Phytophthora capsici and Rhizoctonia solani[J].Canadian Journal of Microbiology,2007,53(2):207-212.[25]PICARD K,TIRILLY Y,BENHAMOU N.Cytological effects of cellulases in the parasitism of Phytophthora parasitica by Pythium oligandrum[J].Applied and Environmental Microbiology,2000,66(10):4305-4314.[26]臧超群,赵奎华,刘长远,等.生防菌株SY286对葡萄霜霉病菌的抑菌效果[J].沈阳农业大学学报,2014,45(2):221-224.

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Last Update: 2019-08-15