|Table of Contents|

Induction of Hairy Root of Uncaria rhynchophylla and Content Determination of Rhynchophylline

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年15
Page:
49-54
Research Field:
Publishing date:

Info

Title:
Induction of Hairy Root of Uncaria rhynchophylla and Content Determination of Rhynchophylline
Author(s):
WU ShunSUN JianchunZHOU KaiWANG HuayingLI TingLIU Jiao
(College of Life Science and Technology,Central South University of Forestry and Technology,Changsha,Hunan 410018)
Keywords:
Uncaria rhynchophyllaASAgrobacterium rhizogeneshairy rootrhynchophylline
PACS:
-
DOI:
10.11937/bfyy.20190144
Abstract:
In order to found the culture system of hairy root for Uncaria rhynchophylla,the effect of different explant,concentration of bacterium,AS concentration,drying time on induction of hairy root was studied by using the sterile seedlings as a material and making Agrobacterium rhizogenes(A4,ATCC15834,R16011) infect these explants.The results showed that the induction rate of hairy root was higher when sterile leaves were used as the explant,preincubated for 3 days,soaked for 30 minutes by OD600=0.6 A4 solution,dried for 1 hour,cultured in hormone-free WPM medium with 100 μmol?L-1 AS,selected on screening medium with 600 mg?L-1 cefminox.The induction rate of hairy root was 53.66%.It was confirmed by PCR analysis that the rooting gene in Agrobacterium rhizogenes was integrated into the nuclear genome for Uncaria rhynchophylla.The content of rhynchophylline increased in some hairy roots after hairy roots were cultured in hormone-free 1/2MS liquid medium for 20 days,in which the highest content of rhynchophylline reached a level of 1.211 mg?g-1 DW and was 2.06 times as much as hook branch.

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Last Update: 2019-08-15