|Table of Contents|

Screening of Bioactive Ligands in Trifolium pratense by Affinity Ultrafiltration Mass Spectrometry

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年17
Page:
102-107
Research Field:
Publishing date:

Info

Title:
Screening of Bioactive Ligands in Trifolium pratense by Affinity Ultrafiltration Mass Spectrometry
Author(s):
HAO Yu; LIU Chunming; LI Sainan; WANG Yueqi; HOU Wanchao; WU Tong
(Central Laboratory,Changchun Normal University,Changchun, Jilin 130032)
Keywords:
Trifolium pratense; biomacromolecules; ultrafiltration; high performance liquid chromatography-electrospray mass spectrometry; semi-preparative liquid chromatography
PACS:
-
DOI:
10.11937/bfyy.20190141
Abstract:
Trifolium pratense extracts were used as test materials,and lactate dehydrogenase(LDH)，xanthineoxidase(XOD) and α-glucosidase were used as biological target molecules.In order to treat stroke and diabetes，the method of receptor-ligand affinity ultrafiltration,ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry (UPLC-Q-Exactive) and semi-preparative liquid chromatography were used to screen active ligands with enzyme inhibition in Trifolium pratense extracts.The results showed that there were six active ligands with enzyme inhibition in the extracts of Trifolium pratens，namely：daidzin，ononin，daidzein，genistein，formononetin and biochanin A.The purity of the six active ligands were separated by semi-preparative liquid chromatography was above 92%，which has potential value in the treatment of stroke and diabetes.

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Last Update: 2019-09-24