|Table of Contents|

Optimization of Embryo Induction System for Isolated Microspore Culture in Carrot

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年13
Page:
68-72
Research Field:
Publishing date:

Info

Title:
Optimization of Embryo Induction System for Isolated Microspore Culture in Carrot
Author(s):
WANG Baosheng; LIAN Yong; ZHANG Yanping; LIU Xiangping; HU Shun; CHEN Yuanmin
(Institute of Vegetable,Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences,Hohhot,Inner Mongolia 010031)
Keywords:
carrot; microspore; embryoid; optimization
PACS:
-
DOI:
10.11937/bfyy.20185051
Abstract:
Ten genotypes of carrot were used to study the effect factors of embryo induction.The results showed that three of the ten genotypes induced embryoids,and the rate of embryogenesis varied significantly among different genotypes,indicating that genotype was an important factor affecting microspore embryo induction.The rate of embryo induction at full flowering stage was significantly higher than that at the other two stages,indicating that full flowering stage was the best time for embryo induction.Among all the experimental conditions,heat shock at 33 ℃ for 2 days had the highest embryogenesis rate,which was the best heat shock condition for microspore embryo induction of carrot.The best ratio of hormone was 0.5 mg?L-1 2,4-D added in the medium.Adding a certain amount of activated carbon to the medium was beneficial to the embryo induction,and the optimum concentration was 0.2 g?L-1.

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Last Update: 2019-08-01