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¡¶±±·½Ô°ÒÕ¡·[ISSN:1001-0009/CN:23-1247/S]

Issue:

2019Äê17

Page:

7-13

Research Field:

Publishing date:

Info

Title:

Identification and Bioinformation Analysis of HSP70 Protein Family in ª«Cucurbita moschataª«

Author(s):

HU Yanping1; ZHOU Yang2; YUN Tianhai1; ZHU Baibi1; WANG Min1; ZHANG Wen1

(1.The Institute of Vegetables,Hainan Academy of Agricultural Sciences,Hainan Key Laboratory of Vegetable Biology,Hainan Vegetable Breeding Engineering Technology Research Center,Haikou,Hainan 571100£»2.College of Horticulture,Hainan University,Haikou,Hainan 570228)

Keywords:

Cucurbita moschataª«; heat shock protein 70; bioinformation; promoter analysis

PACS:

-

DOI:

10.11937/bfyy.20184654

Abstract:

Heat shock protein 70 (HSP70) is a kind of specific stress protein in response to adversity stress in plant.The ª«[STBX]HSP70[STBZ]ª« gene family was identified based on the pumpkin genome database,and the protein characteristics,gene structures,conserved domains and promoter sequences were analyzed by bioinformation methods.The results showed that there were 10 ª«[STBX]HSP70[STBZ]ª« genes in the database.The sequence lengths of HSP70 proteins were varying from 572 to 843 amino acid residues.The HSP70 proteins contained 6-8 conserved domains and could be clustered into four classes.The intron/exon structural analyses revealed that the ª«[STBX]HSP70[STBZ]ª« genes contain different introns varying from 0 to 8.Several ª«cisª«-elements were found in the promoter region of ª«[STBX]HSP70[STBZ]ª« genes,indicating that HSP70 might be involved in the regulation of multiple functions.These results lay a foundation for further study on the function of HSP70 family proteinsy in ª«Cucurbita moschataª«.

References:

[1]YAMORI W,HIKOSAKA K,WAY D A.Temperature response of photosynthesis in C3,C4 and CAM plants:Temperature acclimation and temperature adaptation[J].Photosynthesis Research,2014,119:101-117.[2]GUPTA S C,SHARMA A,MISHRA M,et al.Heat shock proteins in toxicology:How close and how far?¡¼KG-*2¡½[J].Life Science,2010,86(11/12):377-384.[3]SWINDELL W R,HUEBNER M,WEBER A P.Transcriptional profiling of ª«Arabidopsisª« heat shock proteins and transcription factors reveals extensive overlap between heat and non-heat stress response pathways[J].BMC Genomics,2007,8:125.[4]ALTSCHULER M,MASCCARENHAS J P.Transcription and translation of heat shock and normal proteins in seedlings and developing seeds of soybean exposed to a gradual temperature increase[J].Plant Molecular Biology,1985,5:291-297.[5]Ñî¹úÕý,ÕÅÐãÈç,ËïÏæÄþ,µÈ.ÃÞ»¨Èȼ¤µ°°×²úÉú¹æÂɵijõ²½Ñо¿[J].»ªÖÐÅ©Òµ´óѧѧ±¨,1997(16):18-25.[6]Ñî½ðÓ¨,ËïÓ±,Ëï°®Çå,µÈ.·¬ÇѪ«[STBX]LeHsp110/ClpB[STBZ]ª«»ùÒòµÄ·Ö×Ó¿Ë¡¼°Æä¶ÔÖ²ÎïÄÍÈÈÐÔµÄÓ°Ïì[J].ÉúÎ﹤³Ìѧ±¨,2006,22(1):52-57.[7]GROVER A,MITTAL D,NEGI M,et al.Generating high temperature tolerant transgenic plants:Achievements and challenges[J].Plant Science,2013,205:38-47.[8]QUEITSCH C,SANGSTER T A,LINDQUIST S.Hsp90 as a capacitor of phenotypic variation[J].Nature,2002,417:618-624.[9]XU J,XUE C,XUE D,et al.Overexpression of GmHsp90s,a heat shock protein 90 (Hsp90) gene family cloning from soybean,decrease damage of abiotic stresses in ª«Arabidopsis thalianaª«[J].PLoS One,2013,8(7):e69810.[10]TOMINAGA H,COURY D A,AMANO H,et al.cDNA cloning and expression analysis of two heat shock protein genes,ª«[STBX]Hsp90ª« and ª«Hsp60[STBZ]ª«,from a sterile ª«Ulva pertusa ª«(Ulvales,Chlorophyta)[J].Fisheries Science,2012,78(2):415-429.[11]MAIMBO M,OHNISHI K,HIKICHI Y,et al.Induction of a small heat shock protein and its functional roles in ª«Nicotiana ª«plants in the defense response against ª«Ralstonia solanacearumª«[J].Plant Physiology,2014,145(4):1588-1599.[12]SU P H,LI H.ª«Arabidopsisª« stromal 70-kD heat shock proteins are essential for plant development and important for thermotolerance of germinating seeds[J].Plant Physiology,2008,146(3):1231-1241.[13]KIM S R,AN G.Rice chloroplast-localized heat shock protein 70,OsHsp70CP1,is essential for chloroplast development under high temperature conditions[J].Journal of Plant Physiology,2013,170(9):854-863.[14]SONG A,ZHU X,CHEN F,et al.A Chrysanthemum heat shock protein confers tolerance to abiotic stress[J].International Journal of Molecular Science,2014,15:5063-5078.[15]SUN H,WU S,ZHANG G,et al.Karyotype stability and unbiased fractionation in the paleo-allotetraploid ª«Cucurbita ª«genomes[J].Molecular Plant,2017,10:1293-1306.[16]RITOSSA E.A new puffing pattern induced by temperature shock and DNP in ª«Drosophilaª«[J].Experientia,1962,18:571-573.[17]LARGE A T,GOLDBERG M D,LUND P A.Chaperones and protein folding in the archaea[J].Biochemical Society Transaction,2009,37:46-51.[18]SUNG D,VIERLING E,GUY C L.Comprehensive expression profile analysis of the ª«Arabidopsis [STBX]HSP70[STBZ]ª« gene family[J].Plant Physiology,2001,126(2):789-800.[19]³ÂÐñ,ʯÀÝ,Öìè´,µÈ.Ö²ÎïHSP70µ°°×¼Ò×å·Ö×Ó½ø»¯ÌØÕ÷¼°Æä±í´ïģʽ·ÖÎö[J].»ùÒò×éѧÓëÓ¦ÓÃÉúÎïѧ,2017,36(10):4282-4294.[20]ÍõÈÙÇà,Íòºì½¨,ÀîÖ¾åã,µÈ.·¬ÇѪ«[STBX]HSP70[STBZ]ª«»ùÒò¼ø¶¨¼°ÏµÍ³·¢Óý¹Øϵ·ÖÎö[J].ºËũѧ±¨,2014,28(3):378-385.

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Last Update: 2019-09-20