|Table of Contents|

Isolation and Expression Analysis of Cyp450 Genes in Tibet Rhodiola renulata

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年18
Page:
116-127
Research Field:
Publishing date:

Info

Title:
Isolation and Expression Analysis of Cyp450 Genes in Tibet Rhodiola renulata
Author(s):
ZHANG Lipeng; HAN Xiangyan; CHENG Lulu; TENG Yanjiao; YU Deshui; SONG Wenqin
(College of Life Sciences,Nankai University,Tianjin 300071)
Keywords:
Rhodiola crenulata; cyp450; bioinformation; expression analysis
PACS:
-
DOI:
10.11937/bfyy.20184635
Abstract:
Using Tibet Rhodiola crenulata as research materials,the cytochrome p450 (cyp450) gene family members were analyzed by bioinformatics and real-time PCR,which would be helpful to obtain preliminary understanding and fill in the research gap for this gene family.The results showed that the open reading frames of 16 Rccyp450 genes were all above 1 374 bp and the length of amino acid sequence were 480-530.Sequence alignment results showed that Rccyp450 genes belonged to 10 subfamilies,of which 4 genes were A type and 12 genes were non-A type.In addition,Rccyp450 genes contained more than 33 phosphate sites which indicated that they might be modified by phosphatases.The expression pattern for tissue and stresses presented that Rccyp450 genes expressed distinctly differently in three tissues and could be induced by low temperature/UA and three hormones included abscisic acid,auxin and jasmonic acid.

References:

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Last Update: 2019-10-08