|Table of Contents|

Effect of Sampling and Sending Conditions on RT-PCR Detection of Apple Virus Sample

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年19
Page:
13-20
Research Field:
Publishing date:

Info

Title:
Effect of Sampling and Sending Conditions on RT-PCR Detection of Apple Virus Sample
Author(s):
JIANG Dongshuai; XI Nana; YANG Junyu; CUI Liwen; WANG Yanan; CAO Keqiang
(College of Plant Protection,Agricultural University of Hebei，Baoding，Hebei 071001)
Keywords:
apple virus; RT-PCR detection; collection; conservation; sending condition
PACS:
-
DOI:
10.11937/bfyy.20184618
Abstract:
In order to standardize sample collecting and sending for the detection of apple virus.Apple trees infected with (Apple skin scar viroid,ASSVd)，（Apple chlorotic leafspot virus，ACLSV)，（Apple mosaic virus，ApMV)，（Apple stem pitting virus，ASPV)，（Apple stem grooving virus，ASGV) were used as test materials.Using RT-PCR detection technology，the different tissue parts of fruit trees were collected in different months，and the virus samples were stored at different temperatures for different time.The results provide the basis for standardizing the collection，preservation and sending of apple virus detection samples.The results showed that the detection rate of ASSVd，ACLSV，ApMV，ASPV by RT-PCR in October was higher than that in other periods.The detection rate of four viruses by RT-PCR was higher than that of other tissues by using the annual branch skin of the lower part of the canopy as the material.In April，the detection rate of ASGV RT-PCR was higher than that of other tissues，and the detection rate of the upper crown was higher than that of other tissues.When the annual branch skin samples were stored at 4，25 ℃ for 1 day and 3 days，the results of 5 kinds of viruses in three trees were all positive，and the results of three trees except ApMV，were all positive when stored at 4 ℃ for 5 days.Most of the viruses stored for 7 days or more were negative at any test temperature.

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Last Update: 2019-10-09