|Table of Contents|

Rapid Propagation Technique of One-step-seedling Inducing From Young Leaves in Actinidia chinensis cv.‘Chuhong’

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年19
Page:
28-33
Research Field:
Publishing date:

Info

Title:
Rapid Propagation Technique of One-step-seedling Inducing From Young Leaves in Actinidia chinensis cv.‘Chuhong’
Author(s):
MA Guanxue1; ZHONG Wangao1; 2; DUAN Xinyu1; XIE Zixun1; LI Ziming3; ZHANG Yunfeng1; 4
(1.College of Life Sciences,Yunnan Normal University，Kunming,Yunnan 650500；2.Dixu Junior Middle School，Wenshan,Yunnan 663318；3.Liangfeng Agricultural Science and Technology Co.Ltd.，Jianshui，Yunnan 654306；4.Engineering Research Center of Sustainable Development and Utilization of Biomass Energy,Ministry of Education，Kunming,Yunnan 650500)
Keywords:
Actinidia chinensis cv.‘Chuhong’; one-step-seedling; adventitious bud; rooting
PACS:
-
DOI:
10.11937/bfyy.20184549
Abstract:
In order to establish an efficient tissue culture and rapid propagation system for kiwifruit,the young leaves of seedling derived from the aseptic germination of kiwifruit seed of Actinidia chinensis cv.‘Chuhong’ was used as material,the effect of the various concentration combination of plant hormone added to inducing medium on the induce of adventitious bud directly were studied;and the rooting medium for plantlet derived adventitious bud was also optimized.The results showed that in the inducing medium,with 3.0 mg?L-1 6-BA+0.3 mg?L-1 NAA as plant hormone combination,the adventitious bud which could be used for rooting from the young leaves could be induced directly within 40 days,and the adventitious bud inducing frequency could arrived 87.5%.And the optimized rooting medium for plantlet derived from adventitious bud was 1/2MS+0.7 mg?L-1 IBA+30 g?L-1 sucrose,the rooting rate could arrived 100% with the average root number up to 26 per plant.One kiwifruit seed could propagated about 8 million shares seedlings in one year basing the rapid propagation technique established in present experiment，and most of the adventitious bud was formed through the somatic embryogenesis pathway via observation of histological sections.

References:

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Last Update: 2019-10-09