|Table of Contents|

Transformation of Alfalfa With Bar+CP4EPSPS Genes and Resistance Indentification

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年18
Page:
32-39
Research Field:
Publishing date:

Info

Title:
Transformation of Alfalfa With Bar+CP4EPSPS Genes and Resistance Indentification
Author(s):
WANG Yingzhe; WANG Yinan; REN Wei; XU Ankai; LIU Yanzhi; HAO Dongyun
(Institute of Agricultural Biotechnology,Jilin Academy of Agricultural Sciences,Changchun,Jilin 130033)
Keywords:
alfalfa; transformation; Agrobacterium; Bar gene; CP4EPSPS gene
PACS:
-
DOI:
10.11937/bfyy.20184504
Abstract:
In order to improve the herbicide tolerance of alfalfa,Bar gene and CP4EPSPS gene was transfected into ‘Gongnong No.1’ alfalfa using Agrobacterium-mediated method.The five main factors(such as explants，Agrobacterium species，infection concentration，co-culture time，screening concentration) affecting transformation efficiency were studied and established the ideal transformation system.The results showed that the optimal transformation scheme was to select the regenerated seedling leaves as explants，EHA105，bacteria concentration was OD600=0.8，co-cultivation time was 3 days，the concentration of glufosinate was 2 mg?L-1.Target genes were preliminarily proved to integrate into the genome of alfalfa by molecular identification and strip test.Finally，12 transgenic plants were identified by spraying glyphosate.

References:

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Last Update: 2019-09-29