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Title:: (Collage of Forestry and Horticulture，Xinjiang Agricultural University/Key Laboratory of Forestry Ecology and Industry Technology in Arid Region，Education Department of Xinjiang，Urumqi，Xinjiang 830052)

Author(s):: LI Yalan; PAN Cunde; CHEN Hong; (Collage of Forestry and Horticulture，Xinjiang Agricultural University/Key Laboratory of Forestry Ecology and Industry Technology in Arid Region，Education Department of Xinjiang，Urumqi，Xinjiang 830052)

Keywords:: walnut; SRAP-PCR; heat map; multiple regression trees; R language

PACS:: -

DOI:: 10.11937/bfyy.20184462

Abstract:: The 50 walnut germplasms from Xinjiang were used as experimental materials in this study.The genetic diversity of 50 walnut germplasms were analysed by SRAP(Sequence-related amplified polymorphism) markers in order to provide reference for the preservation，identification and comprehensive development of walnut germplasm resources distributed in Xinjiang.The results showed that 16 SRAP primer combinations amplified 178 bands，135 polymorphic bands，75.84% polymorphism ratio.The cluster analysis and cluster sharpness tests showed that the test germplasm was divided into 5 groups，and the heat map displayed different primers were used to distinguish germplasm.Multiple regression trees showed that 12 amplified bands in different primers could distinguish 50 accessions.The above studies indicate that the genetic diversity of the tested walnut germplasm was abundant，and the selected SRAP primer combinations are effective for distinguishing the tested germplasm，among which M4/E4,M7/E5,M14/E4,M2/E11,M3/E4,M2/E4,M12/E13,M7/E1,M14/E13 and M1/E3 ten primer combinations were important primer combinations for the identification of germplasm for testing，which can provide basic data for basic theoretical research and breeding research of walnut germplasm resources.At the same time，the application of R language in the statistical analysis of this paper provides a new data analysis method for the future R language applied to the study of walnut plant morphology and genetic variation.

References:

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(Collage of Forestry and Horticulture，Xinjiang Agricultural University/Key Laboratory of Forestry Ecology and Industry Technology in Arid Region，Education Department of Xinjiang，Urumqi，Xinjiang 830052)(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

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