|Table of Contents|

Rapid Propagation of Disease-free Citrus sinensis Osbeck vc.Newhall Production Using Debacterization and Microshoot tip grafting in vitro

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年18
Page:
26-31
Research Field:
Publishing date:

Info

Title:
Rapid Propagation of Disease-free Citrus sinensis Osbeck vc.Newhall Production Using Debacterization and Microshoot tip grafting in vitro
Author(s):
CHEN Chuanhong; GU Xiaobin; YING Jiaxuan; GAO Bingbing; CHEN Yineng
(School of Chemical Biology and Materials Science，East China Institute of Technology，Nanchang，Jiangxi 330013)
Keywords:
Citrus sinensis Osbeck vc.Newhall; Citrus Huanglongbing; bud proliferation; heat treatment; microshoot tip grafting
PACS:
-
DOI:
10.11937/bfyy.20184424
Abstract:
The effects of disinfection and sterilization methods，plant growth regulator combination，heat treatment temperature and time，and the size of stem tip on the establishment of sterile system，induction and proliferation of cluster buds，debacterization and microshoot tip grafting were discussed for (Citrus sinensis (L.) Osbeck) in Gannan region.The results showed that 83.20% of the viable sterile culture materials could be obtained by sterilizing with 70% alcohol for 30 seconds，washing with sterile water for 3 times，and then sterilizing with 0.1% mercuric chloride and Tween-80 for 15 minutes.Taking MS+6-BA 1.0 mg?L-1+NAA 0.5 mg?L-1 as the optimal bud induction medium，the induction rate reached 89.72%.The best multiplication medium for cluster buds was MS+6-BA 2.0 mg?L-1+NAA 0.4 mg?L-1+GA3 0.1 mg?L-1，with the highest multiplication rate of 394.26%.The survival rate was 63.32% and the bacteria and virus-free rate was 93.21% when the stem tip of 0.6 mm was treated at 40 ℃ for 40 minutes.

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Last Update: 2019-09-29