|Table of Contents|

Cloning of SlHB8 Gene From Tomato and Its Response to Abiotic Stress

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年18
Page:
10-18
Research Field:
Publishing date:

Info

Title:
Cloning of SlHB8 Gene From Tomato and Its Response to Abiotic Stress
Author(s):
YANG Yang1; XIAN Zhiqiang2; CHEN Riyuan1; HAO Yanwei1
(1.College of Horticulture，South China Agricultural University，Guangzhou，Guangdong 510642；2.School of Life Sciences/College of Mathematics and Statistics，Chongqing University，Chongqing 400000)
Keywords:
tomato; SlHB8; HD-Zip III transcription factor; abiotic stress
PACS:
-
DOI:
10.11937/bfyy.20184262
Abstract:
The HD-Zip III transcription factor family plays an important role in plant growth and development.In this study，Micro-Tom tomato was used as material.Using RT-PCR，fluorescence quantitative PCR and bioinformatics methods，the  [STBX]SlHB8 gene′s sequence characteristics，expression patterns and responses under abiotic stress were studied so as to lay a foundation for identifying the function of [STBX]SlHB8 gene in tomatoes.The results showed that there was one gene encoding the HD-Zip III transcription factor (SlHB8) in tomato.The full length of SlHB8 was 2 523 bp，encoding 841 amino acids with four conserved domains of HD，bZip，START and MEKHLA，showing the highest homology with ATHB8 of Arabidopsis thaliana.SlHB8 was expressed in all tomato tissues，and the highest expression was found in stem.All these treatments，such as cold，heat，drought，wound，ethylene(ETH)，and salicylic acid(SA) could reduce the expression level of this gene.Gibberellin(GA)，abscisic acid(ABA)，and auxin treatment could make the expression level of the gene to increase first and then decrease.Promoter sequence analysis by PLACE and PlantCARE revealed that there were many cis-acting elements such as gibberellin(GA)，abscisic acid(ABA)，salicylic acid(SA)，heat shock，wound，and drought.These results indicated that SlHB8 might play an important role in regulating the abiotic stress response of tomato.

References:

[1]张纪涛,马大炜.番茄幼苗对水分胁迫的生理响应[J].北方园艺,2015(7):46-49.[2]吴洪启,罗文巧,赵帅,等.干旱胁迫对番茄叶片蜡质积累的影响[J].西北农林科技大学学报,2017,45(7):73-80.[3]李景富,崔亚男,姜景彬,等.抗旱番茄种质资源筛选及抗旱性评价[J].东北农业大学学报,2018,49(4):1-9.[4]许向阳,陈小玉,赵婷婷,等.外源ABA喷施提高番茄幼苗抗旱性研究[J].东北农业大学学报,2018,49(9):1-8.[5]李金华,王亚玲,潘宇,等.番茄抗旱基因工程研究进展[J].中国蔬菜,2017(12):14-23.[6]王宏,李刚波,张大勇,等.植物 HD-Zip转录因子的生物学功能[J].遗传,2013,35(10):1179-1188.[7]朱莹莹,于亮亮,汪杏芬,等.HD-Zip III转录因子家族与植物细胞分化[J].植物学报,2013,48(2):199-209.[8]秦永芳,李登弟,李学宝.植物HD-Zip转录因子研究进展[J].细胞生物学杂志,2009,31(4):514-520.[9]周岩,何男男,魏琦超,等.同源异型域-亮氨酸拉链蛋白研究进展[J].湖北农业科学,2010,49(11)：2913-2917.[10]ROODBARKELARI F,GROOT E P.Regulatory function of homeodomain-leucine zipper (HD-ZIP) family proteins during embryogenesis[J].New Phytologist,2017,213:95-104.[11]BYRNE M E.Shoot meristem function and leaf polarity:The role of class III HD-Zip genes[J].PLoS Genetics,2006,2(6):e89.[12]LANDAU U,ASIS L,ESHED W L.The ERECTA,CLAVATA and class III HD-Zip pathways display synergistic interactions in regulating floral meristem activities[J].PLoS One,2015,10(5):e0125408.[13]LEE C,CLARK S E.A WUSCHEL-Independent stem cell specification pathway is repressed by PHB,PHV and CNA in Arabidopsis[J].PLoS One,2015,10(5):e0126006.[14]DU Q,WANG H.The role of HD-Zip III transcription factors and miR165/166 in vascular development and secondary cell wall formation[J].Plant Signaling & Behavior,2015,10(10):e1078955.[15]URSACHE R,MIYASHIMA S,CHEN Q,et al.Tryptophan-dependent auxin biosynthesis is required for HD-Zip III-mediated xylem patterning[J].Development,2014,141(6):1250-1259.[16]MERELO P,RAM H,PIA M,et al.Regulation of miR165/166 by class II and class III homeodomain leucine zipper proteins establishes leaf polarity[J].Proc Natl Acad Sci USA,2015,113(42):11973-11978.[17]ZHOU G K,KUBO M,ZHONG R,et al.Overexpression of miR165 affects apical meristem formation,organ polarity establishment and vascular development in Arabidopsis[J].Plant and Cell Physiology,2007,48(3):391-404.[18]JIA X,DING N,FAN W,et al.Functional plasticity of miR165/166 in plant development revealed by small tandem target mimic[J].Plant Science,2015,233:11-21.[19]杨波,罗倩,靳亚军,等.水稻HD-Zip Ⅲ家族成员OsHox10的功能分析[J].天津师范大学学报,2018(5):36-41.[20]ZHANG J,ZHANG H,SRIVASTAVA A K,et al.Knock-down of rice microRNA166 confers drought resistance by causing leaf rolling and altering stem xylem development[J].Plant Physiology,2018,176(3):2082-2094.[21]HU G,FAN J,XIAN Z,et al.Overexpression of SlREV alters the development of the flower pedicel abscission zone and fruit formation in tomato[J].Plant Science An International Journal of Experimental Plant Biology,2014,229:86-95.[22]LIU T,HUANG B,CHEN L,et al.Genome-wide identification,phylogenetic analysis,and expression profiling of polyamine synthesis gene family members in tomato[J].Gene,2018,661:1-10.[23]LI S G,LI W F,HAN S Y,et al.Stage-specific regulation of four HD-ZIP III transcription factors during polar pattern formation in Larix leptolepis somatic embryos[J].Gene,2013,522(2):177-183.[24]KO J H,PRASSINOS C,HAN K H.Developmental and seasonal expression of PtaHB1,a populus gene encoding a class III HD-Zip protein,is closely associated with secondary growth and inversely correlated with the level of microRNA (miR166)[J].New Phytologist,2006,169(3):469-478.[25]陈浣,吕婧,孙玉合.普通烟草 HD-Zip Ⅲ家族全基因组鉴定和表达分析[J]基因组学与应用生物学,2017,36(8):3034-3041.[26]BAIMA S.The Arabidopsis ATHB-8 HD-Zip protein acts as a differentiation-promoting transcription factor of the vascular meristems[J].Plant Physiology,2001,126(2):643-655.[27]ITOH J I,HIBARA K I,SATO Y,et al.Developmental role and auxin responsiveness of class III homeodomain leucine zipper gene family members in rice[J].Plant Physiology,2008,147(4):1960-1975.

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Last Update: 2019-09-29