|Table of Contents|

Tissue Culture and Rapid Propagation of Tripterygium wilfordii Seedlings Based on Air Rooting

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年15
Page:
119-125
Research Field:
Publishing date:

Info

Title:
Tissue Culture and Rapid Propagation of Tripterygium wilfordii Seedlings Based on Air Rooting
Author(s):
JIANG Liangzhen1; MA Jianhua2; HUANG Peng1; WANG Yuehua1; LI Rui1; ZHAO Qi1
(1.College of Pharmacy and Biological Engineering,Chengdu University,Chengdu,Sichuan 610106；2.Sichuan Colorful Forestry Development Co.Ltd.,Bazhong,Sichuan 635600)
Keywords:
Tripterygium wilfordii; tissue culture; rapid proliferation; air suspension rooting; plant hormones
PACS:
-
DOI:
10.11937/bfyy.20184075
Abstract:
To develop new tissue culture and rapid propagation systems of Tripterygium wilfordii Hook.f.,the young shoots were used as explants to induce axillary buds germination.Germinated axillary buds were cut for root inducing and elongation of stems and leaves.The elongated stems were excised into budded stem segments,from which roots were induced in a specific culture medium to achieve rapid plant proliferation.The results showed that the best sterilization effects were achieved when the explants were soaked in 75% alcohol for 30 seconds,washed three times with sterile water,soaked in 0.1% mercury liter solution for 10 minutes.The most effective medium for axillary bud germination was MS medium with 1.5 mg?L-1 6-BA+0.2 mg?L-1 NAA.The efficacy could reach 98.3%.The optimum medium for inducing rapid rooting of stem segments was 1/2MS medium with 0.2 mg?L-1 NAA+0.1 mg?L-1 IAA+0.03% activated carbon.The optimal root-inducing condition was air suspension culture with the lower stem ends of ecology at about 1 cm height from the medium at (23±1)℃,and the humidity being 70%±5% by the 12 h/12 h light/dark culture.The germination efficacy of adventitious roots reached 91.5%.In this study,a tissue culture system for rapid propagation of Tripterygium wilfordii seedlings was established.With this simple method,the monthly proliferation coefficient could reach 3.8.Cutting and transferring budded stem segments to root-inducing medium could realize rapid proliferation of stems and leaves and rooting at the same time.

References:

[1]涂育合.雷公藤栽培与利用[M].北京：中国农业出版社,2006.[2]刘为萍,刘素香,唐慧珠,等.雷公藤研究新进展[J].中草药,2010,41(7):1215-1218.[3]汪群红,胡敏.雷公藤的药理作用与毒副作用[J].中国药业,2010,19(19):85-86.[4]强春倩,刘世任,都本敏.雷公藤药理研究进展[J].中国中医急症,2006,15(2):108-109.[5]薛璟,贾晓斌,谭晓斌,等 雷公藤化学成分及其毒性研究进展[J].中华中医药杂志,2010(5):726-733.[6]LIU Q.Triptolide and its expanding multiple pharmacological functions[J].International Immunopharmacology,2011,11(3):377-383.[7]LI X J,JIANG Z Z,ZHANG L Y.Triptolide：Progress on research in pharmacodynamics and toxicology[J].J Ethnopharmacol，2014,155(1):67-79.[8]李建友,夏素霞,宋少江.雷公藤二萜类化学成分及在研新药的研究进展[J].沈阳药科大学学报,2012(11):901-908.[9]卞晓霞.雷公藤红素药理作用研究进展[J].中国当代医药,2009,16(13):18-19.[10]任献青,鲁静,孟祥乐,等 雷公藤红素药理作用最新研究进展[J].中华中医药杂志,2013(9):2679-2682.[11]林照授,田有圳,涂育合,等.雷公藤苗木的繁育方法[J].林业科技通讯,2013(4):23-25.[12]曾黎辉,吕柳新.木本果树遗传转化研究进展[J].果树学报,2002,19(3):191-198.[13]赖呈纯,余亚白,谢鸿根,等.雷公藤组织培养快繁技术初探[J].武夷科学,2006,22(1):182-184.[14]刘希华,曾淑兰,丁昌俊,等.雷公藤以芽繁芽组织培养研究[J].西南林业大学学报,2009,29(1):35-38.[15]李琰，冯俊涛，陈新雨,等.雷公藤胚性愈伤组织再生植株的增殖及其稳定性[J].林业科学,2009,45(1):57-61.[16]陈凌艳,陈拓,荣俊冬,等.雷公藤组织培养快速繁殖技术研究[J].江苏林业科技,2016,43(2):8-12.[17]孙乙铭,徐建中,王志安,等.HPLC测定不同采收年限雷公藤根及不同部位雷公藤甲素含量[J].中国现代应用药学,2009(11):904-906.

Memo

Memo:
-
Last Update: 2019-08-19