|Table of Contents|

Molecular Identification and Phylogenetic Analysis of Lecidea tessellata

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年14
Page:
40-47
Research Field:
Publishing date:

Info

Title:
Molecular Identification and Phylogenetic Analysis of Lecidea tessellata
Author(s):
Aerguli JAMAHATE; Reym MAMUT
(College of Life Science and Teconology,Xinjiang University,Urumqi,Xinjiang 830046)
Keywords:
lichen; Lecidea tessellata; systematics; phylogeny; Xinjiang
PACS:
-
DOI:
10.11937/bfyy.20183887
Abstract:
In order to indentify cryptic species within Lecidea tessellata,the specimensof Lecidea tessellatadistributed in Xinjiang were seleted as materials.The internal transcribed spacer (ITS) of L.tessellata was amplified specifically to be used for molecular analyses.The cluter analysis of the genetic distances,automatic barcode gap discovery and molecular phylogenies were studied.The results showed that the length of ITS gene fragment was 528 bp,including 62 variation sites,43 reduced sites and 460 conserved sites.The G+C base composition accounted for 56.1% of the total content,and G+C content was higher than A+T content.Haplotype (gene) diversity (Hd) was 0.993,and nucleotide diversity (Pi) was 0.048 76.The topologies of the molecular phylogenies and cluter based on genetic distances were coincident which fell into two clades,and they were identified as two species by the automatic barcode gap discovery approach.The results indicated that there were two species which identified Lecidea tessellata var. tessellata and Lecidea tessellata var.caesia.

References:

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Last Update: 2019-08-12