|Table of Contents|

Micropropagation of Sexual Triploid Hybrid Cymbidium ‘Huanghe’(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年09
Page:
69-75
Research Field:
Publishing date:

Info

Title:
Micropropagation of Sexual Triploid Hybrid Cymbidium ‘Huanghe’
Author(s):
XU Shiying1; LI Yingxue1; WU You1; ZHU Jiao1; GUO Herong1; 2; ZENG Ruizhen1; 2
(1.College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou,Guangdong 510642；2.Guangdong Provincial Key Laboratory of Plant Molecular Breeding,South China Agricultural University,Guangzhou,Guangdong 510642)
Keywords:
hybrid Cymbidium; sexual polyploid; rhizome; micropropagation
PACS:
-
DOI:
10.11937/bfyy.20183835
Abstract:
In order to establish the technical system of micropropagation of sexual triploid hybrid Cymbidium ‘Huanghe’,the test-tube seedlings were employed to investigate the factors influencing on induction,proliferation and differentiation of rhizome,seedling strengthening and transplantation by using of tissue culture rapid propagation method.The results showed that the initial dedifferentiation time of shoot tip with transverse cutting was earlier and the induction rate was higher than that with longitudinal cutting.The length of rhizome,combination of exogenous hormone,light intensity and organic additives had significant effects on rhizome proliferation,and when rhizomes with 0.50 cm in length were inoculated on MS+6-BA 1.00 mg?L-1+NAA 0.20 mg?L-1+potato 80.00 g?L-1+sucrose 30.00 g?L-1+AC 0.30 g?L-1 and cultured at 26 ℃ under 1 000 lx light intensity for 40 days,the proliferation coefficient was the highest (3.18).Combination of exogenous hormone significantly affected rhizome differentiation,and when rhizomes in 1.00 cm length were inoculated on MS+6-BA 1.00 mg?L-1+NAA 0.20 mg?L-1+sucrose 20.00 g?L-1+AC 0.02 g?L-1 and cultured at 26 ℃ under 2 000 lx light intensity for 40 days,the bud and seedling differentiation rates were 60.00% and 57.50%,respectively.When seedlings with 4.0 cm high were inoculated on 1/2MS+6-BA 0.10 mg?L-1+NAA 0.50 mg?L-1+sucrose 20.00 g?L-1+AC 0.50 g?L-1 and cultured at 26 ℃ under 2 000 lx light intensity for 50 days,the average seedling height and mean number of root were 7.32 cm and 3.56,respectively.The transplantation survival rate of seedlings in April and October was 98.67% and 99.67%,respectively by using of moss as culture substance.The above results demonstrate that it was feasible to produce seedlings of Cymbidium ‘Huanghe’ by the use of tissue culture rapid propagation technology.

References:

[1]张志胜.兰花遗传育种[M].2版.北京:中国林业出版社,2010:298-325.[2]王木桂,曾瑞珍,谢利,等.大花蕙兰四倍体的离体诱导和鉴定[J].西北植物学报,2010,30(1):56-62.[3]朱根发,吕复兵,王碧青,等.大花蕙兰品种的染色体数目分析[J].园艺学报,2006,3(2):417-421.[4]王玉英,李光宏,李志敏,等.野生黄蝉兰多倍体诱导初报[J].江苏农业科学,2014(4):132-134.[5]李涵,李慧敏,陆琳,等.杂交兰‘黄金小神童’ 四倍体诱导技术研究[J].西南林业大学学报,2018,32(2):70-75.[6]王木桂，曾瑞珍，谢利,等.墨兰多倍体的离体诱导和鉴定[J].中国农学通报，2011,27(2):132-136．[7]XIE L,ZHOU S S,WANG M G,et al.Creation and micropropagation of polyploids in Cymbidium hybridum[J].Acta Horticulturae,2017,1167:107-114.[8]王园园.四倍体杂交兰遗传性状分析及其抗逆性研究[D].南京:南京农业大学,2013.[9]宋莲,王玉英,张宇欢,等.墨兰绿墨素与大花蕙兰世界和平杂交种组培快繁技术[J].江苏农业科学,2017,45(24):41-43.[10]胡蕾,申婷.杂交兰的组织培养与快繁技术[J].浙江农业科学,2017,58(2):259-260,264.[11]谢利,马晓娟,郭和蓉,等.杂交兰类原球茎增殖中芽分化的控制和快速繁殖[J].植物生理学报,2014,50(2):209-213.[12]左利娟,李志强,郑志勇,等.杂交兰根状茎的增殖与分化成苗技术[J].江苏农业科学,2015,43(6):54-56.[13]杨靖,谢利,曾瑞珍,等.影响 ‘小凤兰’ 根状茎增殖、分化和壮苗的因素研究[J].北方园艺,2016(2):105-109.[14]徐礼谱.玉兔兰组培快繁技术研究[D].广州:华南农业大学,2018.[15]陈云喜,何丹丹,廖浩如,等.影响墨兰×兔耳兰根状茎芽分化的因素[J].中国农学通报,2010,26(9):65-69.[16]OGURA-TSUJITA Y,OKUBO H.Effects of low nitrogen medium on endogenous changes in ethylene,auxins,and cytokinins in in vitro shoot formation from rhizomes of Cymbidium kanran[J].In Vitro Cellular & Developmental Biology-Plant,2006,42(6):614-616.[17]TAO J,YU L Q,KONG F,et al.Effects of plant growth regulators on in vitro propagation of Cymbidium faberi Rolfe[J].African Journal of Biotechnology,2011,10(69):15639-15646.[18]GAO R,WU S Q,PIAO X C,et al.Micropropagation of Cymbidium sinense using continuous and temporary airlift bioreactor systems[J].Acta Physiologiae Plantarum,2014,36(1):117-124.[19]刘祥,孙东玲,卓素卿,等.杂交莲瓣兰根状茎增殖和分化技术研究及分子鉴定[J].江苏农业科学,2018,46(17):156-160.[20]LAVANIA U,SRIVASTAVA S,LAVANIA S,et al.Autopolyploidy differentially influences body size in plants,but facilitates enhanced accumulation of secondary metabolites,causing increased cytosine methylation[J].The Plant Journal,2012,71:539-549.[21]江金兰,叶炜,李永清,等.同源四倍体铁皮石斛的生长及多糖积累[J].植物生理学报,2014,50(4):519-526.[22]彭成力.红掌多倍体资源创建及性状鉴评[D].广州:华南农业大学,2014.

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Last Update: 2019-05-31