|Table of Contents|

Detection of Bacterial Fruit Blotch in Seed-used Pumpkin and Seed Disinfection

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年22
Page:
60-64
Research Field:
Publishing date:

Info

Title:
Detection of Bacterial Fruit Blotch in Seed-used Pumpkin and Seed Disinfection
Author(s):
TIAN JinliWANG ManmanWANG YunliQU ShupingXU WenlongCUI Chongshi
(College of Horticulture and Landscape Architecture,Northeast Agricultural University,Harbin,Heilongjiang 150030)
Keywords:
pumpkinagentiabacterial fruit blotchcontrol efficiencybacteria inhibition
PACS:
-
DOI:
10.11937/bfyy.20183829
Abstract:
Seeds of (Cucurbita maxima) from market were detected for bacterial fruit blotch (BFB) by polymerase chain reaction (PCR) technology.To select the best treatment for seed disinfection,CuSO4,HCl,H2O2,peroxyacetic acid and bleaching powder with different concentrations treated the seeds-borne BFB.The effect of different agentia treatment on the disinfection of pumpkin seed rot pathogen was studied.The results showed that:BFB was detected in 5 different kind of seeds from market.In our study,all 5 treatments with different concentrations had bactericidal effect.However,HCL,H2O2 and peroxyacetic acid also had inhibitory effects on the germination of pumpkin seeds.1.0% CuSO4 with 30 minutes treatment and 1.5% bleaching powder with 30 minutes treatment achieved the best effect on BFB inhibition,and had no effect on seed germination.So,after 1.0% CuSO4 with 30 minutes treatment or 1.5% bleaching powder with 30 minutes treatment,BFB carried by pumpkin seeds could be effectively inhibited.

References:

[1]SCHAAD N W,POSTNIKOVA E,SECHLER A,et al.Reclassification of subspecies of Acidovorax avenae as A.avenae (Manns,1905) emend.,A.cattleyae (Pavarino,1911) comb.nov.,A.citrulli schaad et al.,1978) comb.nov.,and proposal of A.oryzae sp.nov[J].Systematic Applied Microbiology,2008,31(6/8):434-446.[2]WILLEMS A,GOOR M,THIELEMANS S.Transfer of several phytopathogenic pseudomonas species to Acidovorax as Acidovorax avenae subsp.avenae subsp.nov,comb.nov.Acidovorax avenae subsp.citrulli,Acidovorax avenae subsp.cattleyae,and Acidovorax konjaci[J].International Journal of Systematic Bacteriology,1992,42(1):107-119.[3]许勇,张兴平,宫国义,等.细菌性果腐病与瓜类作物健康种子生产及检测技术(上)[J].中国西瓜甜瓜,2003(6):36-37.[4]周黎,丁建军,戴志新.西瓜种子生产中果腐病的防治技术[J].北方园艺,2011(4):166-167.[5]张国裕,张帆,姜立纲,等.西葫芦细菌性果腐病种子带菌检测及消毒[J].中国蔬菜,2012(6):64-67.[6]仇佳伟,廖晓兰.瓜类种子细菌性果斑病菌防治研究进展.生物灾害科学[J].生物灾害科学,2014,37(1):70-73.[7]魏照信,陈荣贤,殷晓燕,等.中国籽用南瓜产业现状及发展趋势[J].中国蔬菜,2013(9):10-13.[8]徐丽珍,于晓凤.黑龙江省籽用南瓜(白瓜籽)科研和生产现状及存在的问题[J].黑龙江农业科学,2009(3):142-143.[9]WALCOTT R R,LANGSTON D B J,SANDERS F H J,et al.Investigating intraspecific variation of Acidovorax avenae subsp.citrulli using DNA fingerprinting and whole cell fatty acid analysis[J].Phytopathology,2000,90(2):191-196.[10]黄月英.西瓜细菌性果斑病的检测与防治[J].福建农业科技,2008(2):62-64.[11]DUTTA B,AVCI U,HAHN M G,et al.Location of Acidovorax citrulli in infested watermelon seeds is influenced by pathway of bacterial invasion[J].Phytopathology,2012,102(5):461-468.[12]HOPKINS D L.Field spread of bacterial fruit blotch of watermelon[J].Phytopathology,1993,83:466.[13]冯志红,李晓丽,宋晓飞,等.不同成熟度对西葫芦种子质量和子叶苗质量的影响[J].种子,2010,29(1):88-89.[14]丁建军,周黎,陈先荣,等.不同药剂对细菌性果腐病的抑菌效果测试初报[J].中国西瓜甜瓜,2005(2):17-18.[15]赵仁君,周志成,冯孝立,等.瓜类细菌性果腐病的防治与健康种子生产[J].中国瓜菜,2008(1):38-39.[16]杜艳媚,王琳,李小妮,等.西瓜细菌性果斑病带菌部位检测及种子处理研究[J].广东农业科学,2011(19):121-123.[17]赵廷昌,孙福在,王兵万,等.药剂处理种子防治哈密瓜细菌性果斑病[J].植物保护,2003,29(4):58-61.[18]宋顺华,郑晓鹰,李丽.西瓜果腐病种子带菌的PCR 检测[J].种子,2007,26(12):24-26.

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Last Update: 2019-12-11