|Table of Contents|

SSR Marker of Black Rot (Xanthommonas campestris pv.campestris) Resistance Gene of Cabbage （Brassica oleracea var.capitata）Turn CAPS Marker

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年21
Page:
7-12
Research Field:
Publishing date:

Info

Title:
SSR Marker of Black Rot (Xanthommonas campestris pv.campestris) Resistance Gene of Cabbage （Brassica oleracea var.capitata）Turn CAPS Marker
Author(s):
LI Jiangli; LI Chengfeng; WANG Chao; ZHANG Xiaoxuan; JIANG Kaixuan; ZHAO Lejie
(College of Horticulture and Landscape,Northeast Agricultural University,Harbin,Heilongjiang 150030)
Keywords:
cabbage; resistant genes for black rot; molecular marker
PACS:
-
DOI:
10.11937/bfyy.20183780
Abstract:
The hybrid F1 hybridized with the cabbage-resistant parent material A21 and the susceptible parent material D12 were used as the experimental material,and the QTL of the black rot resistance gene designed by the research team was studied by molecular marker method.The OL9A03 primer with a linkage distance of 5.1 cmol?L-1 was subjected to 3 replicates.The SSR marker results showed that the target band was 532 in 785 strains,and the target band was not amplified in 253 strains.Then,according to the label,a 93 bp fragment was obtained.Sequence,a total of 3 pairs of CAPS primers were designed by using this sequence,and 2 pairs of CAPS primers showed a target band,and the polymorphism disappeared.The results showed that the SSR marker was successfully transformed into the sequence deliberately extended region (CAPS) marker C001,and 400 strains of F3 generation were used to verify the black rot infection.283 strains of the infected plants showed resistance and 117 strains showed pathogenicity.The results of original SSR marker and new CAPS marker are consistent and can be used for the purpose of molecular marker-assisted breeding.

References:

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Last Update: 2019-12-05