|Table of Contents|

Genetic Diversity of EST-SSR Markers in Forsythia suspensa (Oleaceae) From Different Habitats

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年13
Page:
140-147
Research Field:
Publishing date:

Info

Title:
Genetic Diversity of EST-SSR Markers in Forsythia suspensa (Oleaceae) From Different Habitats
Author(s):
SHEN Penglong1; DONG Chengming1; XIA Wei2; LI Man1
(1.College of Pharmacy，Henan University of Traditional Chinese Medicine，Zhengzhou，Henan 450046；2.Increase (Tianjin) Innovative Medicine Research Co.Ltd.，Tianjin 300000)
Keywords:
Forsythia suspensa; EST-SSR; genetic diversity; genetic similarity
PACS:
-
DOI:
10.11937/bfyy.20183742
Abstract:
The genetic diversity of 77 Forsythia suspensa germplasm resources collected from 12 areas of Henan and Shanxi Provinces was analyzed by EST-SSR molecular marker technique，in order to explore the genetic similarity of Forsythia suspensa in different habitats and its key influencing factors.The results showed that 14 pairs of polymorphic EST-SSR primers with clear amplified bands and good stability were screened from 60 pairs of primers.14 pairs of primers amplified 370 bands in 77 samples，including 368 polymorphic bands，and the percentage of polymorphic sites was 99.46%.The genetic distances of the 12 areas ranged from 0.020 3 to 0.092 2，and the average genetic distance was 0.055 8.According to genetic similarity coefficient，12 populations were clustered into 5 groups at 0.935 sites and 77 samples were clustered into 5 classes at 0.935 sites.The result of individual clustering was similar to that of origin.The analysis of clustering results showed that the genetic similarity of Forsythia suspensa had no significant correlation with geographical distance，which was mainly related to growth environment and altitude，etc.The experimental results reveal the key factors of genetic similarity of Forsythia suspensa in different habitats,which lays a foundation for the identification of Forsythia suspensa susceptibility and the protection and development of Forsythia resources.

References:

[1]滕训辉.山西野生连翘资源保护与可持续利用研究[J].中国医药导报，2010，7(34)：81-82，115.[2]郭丁丁,张潞,朱秀峰.中药连翘种质资源调查报告[J].时珍国医国药，2012，23(10)：2601-2603.[3]崔旭盛,李鑫,王伟,等.连翘适宜采收期研究[J].安徽农业科学，2017，45(11)：107-108，137.[4]阎新佳,温静,项峥,等.连翘的化学成分研究[J].中草药，2017，48(4)：644-647.[5]全云云,袁岸,龚小红,等.连翘抗炎药效物质基础筛选研究[J].天然产物研究与开发，2017，29(3)：435-438，471.[6]LANG Y,WANG M,XIA J，et al.Effects of soil drought stress on photosynthetic gas exchange traits and chlorophyll fluorescence in Forsythia suspensa[J].Journal of Forestry Research，2018，29(1)：45-53.[7]XIA Y,YANG B,LIANG J，et al.Caffeoyl phenylethanoid glycosides from unripe fruits of Forsythia suspensa[J].Chemistry of Natural Compounds，2015，51(4)：656-659.[8]PAN X,CAO X,LI N，et al.Forsythin inhibits lipopolysaccharide-induced inflammation by suppressing jak-stat and p38 mapk signalings and ros production[J].Inflammation Research，2014，63(7)：597-608.[9]孟南,苏芳,李永.鸡公山和桐柏山连翘遗传多样性分析[J].河南科学，2011，29(3)：297-300.[10]李永,杨攀,李永华.伏牛山区连翘遗传多样性研究[J].西北植物学报，2011，31(4)：665-670.[11]FU Z,LI Y H,LI Y，et al.Isolation and characterization of microsatellite markers for Forsythia suspensa (Thunb.)Vahl (Oleaceae) using 454 sequencing technology[J].Biochemical Systematics and Ecology，2014，57：328-331.[12]FU Z,LEI Y,PENG D，et al.Population genetics of the widespread shrub Forsythia suspensa (oleaceae) in warm-temperate china using microsatellite loci：Implication for conservation[J].Plant Systematics and Evolution，2016，302(1)：1-9.[13]王玲玲,陈东亮,黄丛林,等.SSR分子标记技术在植物研究中的应用[J].安徽农业科学，2017，45(36)：123-126，130.[14]贺润丽,樊杰,平莉莉,等.大豆基因组SSR和EST-SSR在黄芪中的通用性分析[J].分子植物育种，2015，13(5)：994-998.[15]刘果,谢耀坚,陈鸿鹏,等.桉树Genomic-SSR和EST-SSR分子标记的遗传差异性分析[J].桉树科技，2017，34(3)：1-8.[16]张亚东,彭婵,李振芳,等.基因组SSR与EST-SSR标记在杨树不同种间的遗传差异[J].东北林业大学学报，2011，39(12)：8-11，117.[17]王兴春,谭河林,陈钊,等.基于RNA-Seq技术的连翘转录组组装与分析及SSR分子标记的开发[J].中国科学(生命科学)，2015，45(3)：301-310.[18]王竹林.遗传学实验指导[M].杨凌:西北农林科技大学出版社，2011：70-73.[19]强海平,余国辉,刘海泉,等.基于SSR标记的中美紫花苜蓿品种遗传多样性研究[J].中国农业科学，2014，47(14)：2853-2862.[20]汤正辉,祝亚军,谭晓风,等.河南连翘种群遗传多样性的ISSR分析[J].中南林业科技大学学报，2013，33(8)：32-37.[21]孟令芝.基于高通量测序技术的连翘转录组分析及SSR分子标记的开发[D].晋中：山西农业大学，2014.[22]葛亮,袁素霞,王春城,等.百合部分种及品种系统进化关系的EST-SSR标记分析[J].园艺学报，2012，39(11)：2189-2198.[23]邵千顺,高磊,南雄雄,等.利用SSR技术对十七份枸杞材料进行遗传多样性分析及标准指纹图谱构建[J].北方园艺，2015(12)：91-95.

Memo

Memo:
-
Last Update: 2019-08-02