|Table of Contents|

Building and Optimizing the Tissue Culture System of Sequoia sempervirens

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年06
Page:
96-101
Research Field:
Publishing date:

Info

Title:
Building and Optimizing the Tissue Culture System of Sequoia sempervirens
Author(s):
XIE Zhongli1; 2; LI Dan1; YU Xiangjun1; CHEN Yuanshu1; GU Xu1; HE Chengzhong2
(1.Yunnan Academy of Biodiversity,Southwest Forestry University,Kunming，Yunnan 650224;2.Key Laboratory for Forest Genetic and Tree Improvement & Propagation in Universities of Yunnan Province,Southwest Forestry University,Kunming，Yunnan 650224)
Keywords:
Sequoia sempervirens; tissue culture; optimization; proliferation coefficient
PACS:
-
DOI:
10.11937/bfyy.20183728
Abstract:
One-year-old Sequoia sempervirens stem with buds were chosen as explants,by the basic medium and different plant growth regulator levels of contrast test to optimize the ratio of tissue culture of S.sempervirens callus induction,obtaining optimized culture medium of adventitious buds induction,proliferation,rooting and other several growth stages of the S.sempervirens tissue culture.The establishment of S.sempervirens tissue culture system was to improve the reproduction capacity and increase the S.sempervirens,and provide a certain theoretical basis and scientific basis for the rapid tissue culture of S.sempervirens.The results indicated that the best disinfection method was to use 75% alcohol to disinfect for 30 seconds,rinse with sterile water twice,disinfect with 0.1% mercury bichloride for 10 minutes,and rinse with sterile water four times.The optimum medium for the adventitious shoot induction was MS+6-BA 1.0 mg?L-1+NAA 0.10 mg?L-1+sucrose 30 g?L-1+agar 6.0 g?L-1,the induction efficiency of regenerated buds was the best,and the induced buds rate was as high as 2 153.3%.The formula for the optimal adventitious shoot proliferation medium was MS+6-BA 1.0 mg?L-1+NAA 0.10 mg?L-1+sucrose 30 g?L-1+agar 6.0 g?L-1,the proliferative coefficient of adventitious shoots was as high as 15.8.The optimum culture medium for rooting induction was 1/2 MS+KT 1.5 mg?L-1+IBA 1.0 mg?L-1+sucrose 30 g?L-1+agar 6.0 g?L-1,the rooting rate was 90.0%,more root and longer length,and the plant was tall and strong.The suitable transplanting substrate was Vscoria∶Vraw soil∶Vhumus=1∶1∶1 and the survival rate of the plantlets was 91.7%.

References:

[1]郑万钧.中国树木志:第1卷[M].北京:中国林业出版社,1983.[2]李火根,李博,周玉珍,等.北美红杉扦插生根的影响因素分析[J].林业工程学报,2006,20(6):35-37.[3]左显东,祁荣频,王懿祥,等.北美红杉在我国的引种及其生态适应性[J].西部林业科学,2000(4):36-40.[4]左显东,白尚斌,邵金平,等.北美红杉在云南的引种成效及其造林展望[J].西部林业科学,2003(3):2-10.[5]LIU C,XIA X,YIN W,et al.Shoot regeneration and somatic embryogenesis from needles of redwood (Sequoia sempervirens (D.Don.) Endl.)[J].Plant Cell Reports,2006,25(7):621-628.[6]胡庆,段晓毛,吴雪峰,等.北美红杉的组织培养[J].江西林业科技,2003(5):10-12[7]陈芳,马显达,陈娟.北美红杉组培苗工厂化育苗技术[J].西部林业科学,2003(3):11-13.[8]陈芳,马显达,陈娟,等.北美红杉优良无性系组培快繁技术研究[J].林业科学研究,2005,18(1):102-105.[9]董林娜,路群,周根余.北美红杉无性快繁技术的研究[J].上海农业学报,2005,21(4):67-70.[10]SUL I W,KORBAN S S.Direct shoot organogenesis from needles of three genotypes of Sequoia sempervirens[J].Plant Cell Tissue & Organ Culture,2005,80(3):353-358.[11]彭绿春,苏艳,王丽花,等.北美红杉嫩枝的标准化离体繁殖技术研究[J].中国农学通报,2010,26(4):89-92.[12]周元超,徐凌彦,于进英,等.北美红杉组织培养技术研究[J].西部林业科学,2010,39(4):48-51.[13]RIBERIRO J M,TEIXEIRA S L,BASTOS D C.Cultivo in vitro de Sequoia sempervirens L.em meio de nutritivo esterilizado com hipoclorito de sódio[J].Ciência Florestal,2011,21(1):77-82.[14]包慈华,马以凤,刘静芙,等.海岸红杉组织培养诱导完整植株的初步研究[J].科学通报,1979,24(7):321-323.[15]郭达初.北美红杉的器官发生与繁殖[J].植物生理学报,1980(1):95-100.[16]耿云芬.北美红杉扦插育苗试验初报[J].西部林业科学,2001(4):28-30.[17]曾勇,李双龙,向伟,等.1年生北美红杉嫩枝扦插技术研究[J].湖北林业科技,2015(6):4-7.[18]王斐,梅丽,琚淑明.我国北美红杉繁殖技术研究现状[J].中国园艺文摘,2010,26(11):47-48.[19]宋杰,丁鲲,龙江,等.北美红杉种子萌发特性研究[J].西南农业学报,2012,25(4):1427-1431.[20]陈传红，周亚平，余志坚，等.金边虎尾兰组织培养的研究[J].江苏农业科学,2017,45（14）:34-36.[21]尹丽莎,辛雅萱,李斌,等.我国北美红杉组织培养研究进展[J].现代园艺,2016(7):13-15.[22]KORBAN S S,SUL I W.Micropropagation of Coast Redwood (Sequoia Sempervirens)[J].Protocols for Micropropagation of Woody Trees & Fruits,2007，53:23-32.[23]MONFORT L E F,BERTOLUCCI S K V,LIMA A F,et al.Effects of plant growth regulators,different culture media and strength MS on production of volatile fraction composition in shoot cultures of Ocimum basilicum[J].Industrial Crops & Products,2018,116:231-239.[24]琚淑明,高明侠,徐德兰.耐寒北美红杉无性快繁技术研究[J].林业实用技术,2009(1):23-26.[25]赵玉洁,谭彬,李洪涛,等.石榴组织培养及遗传转化技术研究进展[J].河南农业科学,2017,46(4):1-5.[26]黄烈健,王鸿.林木植物组织培养及存在问题的研究进展[J].林业科学研究,2016,29(3):464-470.[27]秦秀兰,庞惠仙,王少龙.四倍体刺槐组织培养研究[J].西部林业科学,2017(4):31-34.

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Last Update: 2019-04-18