|Table of Contents|

Creation and Phenotypic Analysis of Tomato EMS Mutant Library

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年07
Page:
1-9
Research Field:
Publishing date:

Info

Title:
Creation and Phenotypic Analysis of Tomato EMS Mutant Library
Author(s):
ZHANG Ping; JIANG Jingbin; YANG Huanhuan; ZHAO Tingting; XU Xiangyang; LI Jingfu
(School of Horticulture and Landscape Architecture,Northeast Agricultural University,Harbin,Heilongjiang 150030)
Keywords:
tomato; ethyl methyl sulfonate mutagenesis; mutant library; SSR
PACS:
-
DOI:
10.11937/bfyy.20183698
Abstract:
The seeds of tomato variety ‘14637’ were used as materials,the seeds were treated with 0.8% methyl sulfonate (EMS) for 12 hours,and then the phenotypic variation was observed in the leaf,flower,stem,fruit,seed and the like in the group of M1 and M2 population groups,and the analysis and evaluation of the typical mutant strain of the M2 typical mutation were analyzed by 20 pairs of SSR primers,and the tomato mutant library was constructed in order to provide reference for obtaining new tomato germplasm resources.The results showed that the M1-generation population had some variation,such as plants dwarfing,leaf color variation,flower malformation,stem thinness and fruit malformation,but the phenotypic variation was not obvious.A total of 65 variation types were found in the selection of tomato communities of M2 generation,and 189 mutant plants were found with a mutation rate of 12.89%,among which 8 beneficial mutation types were obtained.The variation of the genome level of 13 typical mutant plants in the M3 generation was tested.By SSR primer analysis,it was found that all 13 mutants had variation at the DNA level.This study preliminarily constructed a library of tomato mutant containing 189 strain M2 plants and 65 phenotypic variation.

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Last Update: 2019-04-30