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Effects of Drought on Seed Germination and Seedling Growth of Transgenic AtCuZnSOD Kale(PDF)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年12
Page:
76-82
Research Field:
Publishing date:

Info

Title:
Effects of Drought on Seed Germination and Seedling Growth of Transgenic AtCuZnSOD Kale
Author(s):
YANG Yong1HAI Jun2PANG Keqin1LI Hongyan3
(1.Garden Center of Huanghuai University,Huanghuai University,Zhumadian,Henan 463000;2.Green Department of Zhumadian,Zhumadian,Henan 463000;3.School of Biotechnology and Food Engineering,Huanghuai University,Zhumadian,Henan 463000)
Keywords:
kaleAtCuZnSOD genetransgenic plantdrought tolerance
PACS:
-
DOI:
10.11937/bfyy.20183680
Abstract:
Brassica oleracea was used as test material,drought tolerance of transformed kale with Arabidopsis thaliana L.copper/zinc superoxide dismutase (AtCuZnSOD) gene was studied by Agrobacterium-mediated,PCR,Southern blot and qRT-PCR,in order to increase the germplasm resources of resistance breeding in Kale.The results showed that AtCuZnSOD gene was integrated into the genome of kale.The expression level of AtCuZnSOD in transgenic plants increased significantly.Under PEG-4000 stress,the seed germination potential and germination rate of transgenic AtCuZnSOD kale were higher than those of control.Under drought stress,MDA content of transgenic seedlings was significantly lower than that of wild plants,while POD and CAT activities were higher than those of wild plants.Meanwhile,the increase of average main root length,survival rate and chlorophyll content of transgenic plants were significantly higher than that of wild plants,while dry weight/fresh weight was significantly lower than that of wild plants.The results showed that the drought tolerance of transgenic kale plants with AtCuZnSOD gene was significantly improved.

References:

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Last Update: 2019-07-12