|Table of Contents|

Effect of Sodium Azide and Ethyl Methyl Sulfonate Combined Mutation on Salt Tolerance of Lilium henryi

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年11
Page:
80-85
Research Field:
Publishing date:

Info

Title:
Effect of Sodium Azide and Ethyl Methyl Sulfonate Combined Mutation on Salt Tolerance of Lilium henryi
Author(s):
TIAN Xin; ZHONG Cheng; LI Xingyuan
(College of Comprehensive Health,Kaili University,Kaili,Guizhou 556011)
Keywords:
Lilium henryi; compound mutation; NaN3; EMS; salt tolerance
PACS:
-
DOI:
10.11937/bfyy.20183630
Abstract:
Lilium henryi was used as the test material,and 9 different medium were used for inoculation.The optimal formula for inducing callus was MS+6-BA 1.0 mg?L-1+NAA 0.1 mg?L-1.According to the formula of the callus induced mutation material,successively with NaN3 (1,2,3 mmol?L-1) processing (3,4,5 hours),EMS (0.2%,0.4%,0.6%) treatment (1,2,3 hours) callus and differentiation of mutagenic treatment of adventitious buds treated with 0.4% NaCl stress 0-12 days to salt stress screening,in order to obtain the best resistance to salt concentration compound mutation after processing.The results showed that NaN3 treated with 3 mmol?L-1 was semi-lethal at 3 hours and the mutagenesis rate was 46.7%,and the mutagenesis rate was up to 53.3% with 0.4%EMS.The changes of SOD activity,POD activity,soluble sugar content and soluble protein content in the mutagenesis were higher than those in the control group.Therefore,the metabolism of Lilium henryi was higher,showing its adaptability to salt stress.

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Last Update: 2019-07-09