|Table of Contents|

Construction of Fingerprint of Viburnum Bean Based on SSR Marker

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年09
Page:
1-7
Research Field:
Publishing date:

Info

Title:
Construction of Fingerprint of Viburnum Bean Based on SSR Marker
Author(s):
CHEN Qifu; LI Yanmei; LI Jiayin; HU Yanqiu; WANG Huanli; FENG Guojun
(Crop Research Institute,Heilongjiang University,Harbin,Heilongjiang 150080)
Keywords:
SSR; snap beans; fingerprint; cluster analysis
PACS:
-
DOI:
10.11937/bfyy.20183573
Abstract:
The 14 bean cultivars were used as materials,14 SSR molecular markers were used to study the genetic diversity and specific fingerprints were established,in order to establish a new reference for the identification of snap beans in the future,it also provided a reference method of molecular biology for identifying and classifying the germplasm resources of the snap beans.The results showed that 18 of 74 pairs of SSR primers showed polymorphisms in snap bean samples,and 5 SSR primers（BM200，PVBR269，PVBR6，BM141，BM154） were selected for fingerprint establishment and genetic cluster analysis of 14 bean varieties.The results of clustering showed that 14 snap bean cultivars were divided into two groups at the similarity coefficient of 0.64.The clustering results also suggested that the genetic distance both of ‘Heizhenzhu’ and ‘Huangjingou’ was the closest,with a similarity coefficient of 0.98.To construct the fingerprint of the snap beans had a great useful value for identifying the germplasm resources of the snap beans,the selection of parent,increasing the efficiency of breeding and intellectual property protection.

References:

[1]SCHOONHOVEN A,VOYSEST O.Common beans:Research for crop improvement[M].UK:Redwood Press Ltd,1991:7-8.[2]王中华.DNA指纹图谱技术及其在作物品种资源中的应用[J].分子植物育种,2006,4(3):425-430.[3]刘春良.普通菜豆生长习性相关基因定位[D].北京：中国农业科学院,2016.[4]BUSO G S C,AMARAL Z P S,BRONDANI R P V,et al.Microsatellite markers for the common bean,Phaseolus vulgaris[J].Molecular Ecology Resources,2006,6(1):252-254.[5]YU K,PARK S J,POYSA V,et al.Integration of simple sequence repeat (SSR) markers into a molecular linkage map of common bean(Phaseolus vulgaris L.)[J].Journal of Heredity,2000,91(6):429.[6]GRISI M C,BLAIR M W,GEPTS P,et al.Genetic mapping of a new set of microsatellite markers in a reference common bean (Phaseolus vulgaris) population BAT93×Jalo EEP558[J].Genetics & Molecular Research,2007,6(3):691.[7]BLAIR M W,PEDRAZA F,BUENDIA H F,et al.Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.)[J].Theoretical & Applied Genetics,2003,107(8):1362-1374.[8]ANDERSON J A,CHURCHILL G A,AUTRIQUE J E,et al.Optimizing parental selection for genetic linkage maps[J].Genome,1993,36(1):181-186.[9]ZHANG X,BLAIR M W,WANG S.Genetic diversity of Chinese common bean (Phaseolus vulgaris L.) landraces assessed with simple sequence repeat markers[J].Theoretical & Applied Genetics,2008,117(4):629-640.[10]GAITN-SOLS E,DUQUE M C,EDWARDS K J,et al.Microsatellite repeats in common bean (Phaseolus vulgaris)[J].Crop Science,2002,42(6):2128-2136.[11]CHEN C，XIA R，CHEN H，et al.TBtools,a toolkit for biologists integrating various HTS-data handlingtools with a user-friendly interface[J/OL].http://doi.org/10.1101/289660,2018-03-27/2018-10-04.[12]生信札记.什么，热图工具还能快速进行进化树节点分类可视化？〖KG-*2〗[EB/OL].http://https://www.jianshu.com/p/529ee2e33286,2018-09-29/2018-10-04.[13]邵千顺,高磊,南雄雄,等.利用SSR技术对十七份枸杞材料进行遗传多样性分析及标准指纹图谱构建[J].北方园艺,2015(12):91-95.[14]钱刚,平军娇,王大忠,等.SSR多态性信息含量(PIC)对大麦糖化力的选择效果[J].广东农业科学,2011,38(14):120-123.[15]HILDE N,KURT W,BJRN R.DNA fingerprinting in botany:Past,present,future[J].Investigative Genetics,2014,5(1):1.[16]BLAIR M W,BUENDA H F,GIRALDO M C,et al,Characterization of AT-rich microsatellites common bean (Phaseolus vulgaris L.)[J].Theoretical and Applied Genetics,2008,118:91-103.[17]CHEN M L,WU J,WANG L F,et al.Development of mapped simple sequence repeat markers from common bean (Phaseolus vulgaris L.) based on genome sequences of a Chinese landrace and diversity evaluation[J].Molecular Breeding,2014,33(2):489-496.[18]王庆彪,张扬勇,庄木,等.中国50个甘蓝代表品种EST-SSR指纹图谱的构建[J].中国园艺文摘,2016,47(1):111-121.[19]徐海风,杨加银,程保山.26份菜用大豆品种(系)指纹图谱的构建及其遗传多样性分析[J].江苏农业科学,2014,42(5):145-148.

Memo

Memo:
-
Last Update: 2019-06-03