|Table of Contents|

Determination of Echinacoside in Introducing Plant of Echinacea purpurea

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年09
Page:
119-124
Research Field:
Publishing date:

Info

Title:
Determination of Echinacoside in Introducing Plant of Echinacea purpurea
Author(s):
XU Shijuan1; LU Haixiao2; HUANG Huaxi2; CHEN Rong2; 3; ZHU Changsan4
(1.College of Science，Honghe University,Mengzi,Yunnan 661100;2.College of Biological and Pharmaceutical Sciences,Yulin Normal University,Yulin,Guangxi 537000;3.College of Chinese Medicinal Materials,Jilin Agricultural University,Changchun,Jilin 130118;4.Guangxi Zhuang Autonomous Region Forestry Research Institute,Nanning,Guangxi 530002)
Keywords:
Echinacea purpurea; echinacoside; high performance liquid chromatography(HPLC)
PACS:
-
DOI:
10.11937/bfyy.20183502
Abstract:
The test materials were the the root,stem,leaf and head inflorescence of Echinacea purpurea,which was introduced and cultivated in Mengzi city,Yunnan Province.The quantitative analysis method of echinacoside in Echinacea purpurea was studied by using the method of high performance liquid chromatography(HPLC).The quantitative analysis method of HPLC of determining echinacoside in Echinacea purpurea were optimized and investigated.The results showed that there was in a good linear relationship with the peak area in the range of 0.021 to 0.321 μg.The average recovery rate was 93.24%,and the RSD value was 1.28%.The content of echinacoside was in the range of 0.075%-0.221% in different parts of Echinacea purpurea,and the content of echinacoside in leaves and capitulum was significantly higher than that in roots and stems.The determination method with good precision and reproducibility was reliable,and could be used for the quality evaluation of echinacoside in Echinacea purpurea.The content of echinacoside in Echinacea purpurea for cultivated in Mengzi city,Yunnan Province was high,which is superior to foreign origin and domestic reported cultivation areas.

References:

[1]肖培根.国际流行的免疫调节剂紫锥菊及其制剂[J].中草药,1996,27(1):46-48.[2]李继任,赵玉英,艾铁民.三种松果菊化学成分与生物活性研究进展[J].中国中药志,2002,27(5):334-337.[3]WILLS R B,STUART D L.Effect of handling and storage on alkylamides and cichoric acid in Echinacea purpurea[J].Journal of the Science of food & Agriculture,2000,80:1402-1406.[4]刘一兵.紫锥菊属植物制剂的化学、免疫作用与临床[J].国外医药(植物学分册),2001,16(2):47-54.[5]陈荣,杨跃生,吴鸿.广州地区紫锥菊引种与露地栽培试验初报[J].广东农业科学,2011,38(10):20-22.[6]陈荣,吴鸿.微肥对紫锥菊产量及种子生产的影响[J].中草药,2007,38(9):1400-1403.[7]韩琳娜.紫锥菊遗传多样性的SRAP分析[J].山东农业科学,2014,46(5):60-62.[8]CHEN R,JIANG W Z,LI Q L,et al.Comparison of seven colchicine-induced tetraploid clones with their original diploid clones in purple coneflower(Echinacea purpurea L.)[J].Euphytica,2016,207(2):387-399.[9]李洋,洪莉,刘成,等.松果菊苷药理作用研究进展[J].医药导报,2017,36(1):56-60.[10]LIVERSEY B C,AWANG J F,ARNASON D V,et al.A quantitative HPLC method for the quality assurance of Echinacea products on the North American market[J].Phytochemical Analysis,2000（11）:207-215.[11]LUO X B,CHEN B,YAO S H,et al.Simultaneous analysis of caffeic acid derivatives and alkamides in roots and extracts of Echinacea purpurea by High-performance liquid chromatography-photodiode array detection electrospray mass spectrometry[J].Journal of Chromatography A,2003,986(1):73-81.[12]曹岚,刘桂琴,王勇,等.反相高效液相色谱法测定紫锥菊中松果菊苷的含量[J].现代仪器,2005(2):25-26.[13]陈银花,周旻.样品测定中加标量的确定及加标回收率计算中注意事项的探讨[J].污染防治技术,2016(6):75-77.[14]BAYER R.Content of cehinacoside in Echinacea roots of different origin[J].Beitr Zuechtungstorsch,1996，2(1):64-67.[15]BINNS S E,LIVESEY J F,ARMASON T,et al.Phytochemical variation in Echinacea from roots and flowerheads of wild and cultivated populations[J].Journal of Agricultural & Food Chemistry,2002,50:3673-3687.[16]闫晓慧.狭叶松果菊组织培养、毛状根诱导及其松果菊苷和绿原酸积累的研究[D].重庆:西南大学,2007.[17]吴春华,黄韬,崔锡花,等.白色紫锥菊不定根诱导及咖啡酸衍生物积累研究[J].中国中药杂志,2012,37(24):3768-3772.

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Last Update: 2019-06-11