|Table of Contents|

Cloning and Bioinformatic Analysis of MdLsi1 Gene in Apple (Malus domestica Borkh.)

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年09
Page:
27-33
Research Field:
Publishing date:

Info

Title:
Cloning and Bioinformatic Analysis of MdLsi1 Gene in Apple (Malus domestica Borkh.)
Author(s):
LIU Haili; YANG Leilei; XIN Miaomiao; LIU Jingying
(College of Life Sciences,Northwest A&F University/Shaanxi Key Laboratory of Apple,Yangling,Shaanxi 712100)
Keywords:
apple; silicon transporter gene; promoter; bioinformatics
PACS:
-
DOI:
10.11937/bfyy.20183232
Abstract:
‘Qinguan’ apple (Malus domestica Borkh.) leaves were used as materials,MdLsi1 gene was cloned by RT-PCR method and characterized using bioinformatic analysis,in order to provide an important basis for the molecular mechanism of silicon as an beneficial element during apple tree growth.The results showed that the full-length ORF of MdLsi1 was 873 bp and encoded 290 amino acid residues,a 30.456 kDa protein.The MdLsi1 showed the characteristic features reported as silicon transporter,which had six transmembrane domains,two conserved NPA motifs,a G-S-G-R ar/R selectivity filter and the 108 aa spacing between NPA motifs.The 1 500 bp upstream region of MdLsi1 was analyzed using PlantCARE databases to identify the presence of various cis-regulatory elements.There were cis-acting elements involved different defense and stress responsiveness besides common cis-acting element in the MdLsi1 promoter.The results suggested that MdLsi1 played an important role in silicon uptake and stress response for apple plant.

References:

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Last Update: 2019-06-03