|Table of Contents|

Hairy Root Induction of Pterocephalus hookeri and Screening of Medium

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年18
Page:
110-115
Research Field:
Publishing date:

Info

Title:
Hairy Root Induction of Pterocephalus hookeri and Screening of Medium
Author(s):
XU Yuanjiang1; 2; LIU Jiang1; 3; SU Yanjie1; 3; QUAN Hong2; ZHENG Weilie2; LAN Xiaozhong1; 3
(1.TAAHC-SWU Medicinal Plants Joint R&D Centre,Tibet Agriculture and Animal Husbandry University，Nyingchi,Tibet 860000；2.Res,Institute of Tibet Plateau Ecology,Tibet Agriculture and Animal Husbandry University，Nyingchi,Tibet 860000；3.Food Science College,Tibet Agriculture and Animal Husbandry University，Nyingchi,Tibet 860000)
Keywords:
Pterocephalus hookeri; hairy root; medium; oleanolic acid; ursolic acid; HPLC
PACS:
-
DOI:
10.11937/bfyy.20183019
Abstract:
This study was designed to screen for the optimal medium for root growth of (Pterocephalus hookeri (C.B.Clarke) Hock.).The sterile seedling of Pterocephalus hookeri was used as the research object.The roots were obtained by infecting the leaves with Agrobacterium tumefaciens C58C1 (pRiA4) to study the effects of different infestation conditions on the number of hairy roots.The effects of five mediums (MS,1/4MS,B5,1/2B5 and WPM) on the root biomass and content of oleanolic acid and vrsolic acid for Pterocphalus hookeri were veriflied by the determination of fresh weight、dry weight、oleanolic acid、ursolic acid content in liquid culture.The results showed that the induction rate of Pterocephalus hookeri was the highest (12%) for infect 8 minutes，co-culture 1 day,and the root biomass was the highest in 1/4MS liquid medium.The fresh weight was 9.210 g and the dry weight was 0.529 g.The fresh weight of B5 medium was 5.270 g and the dry weight was 0.385 g.The roots of oleanolic acid and ursolic acid content were the highest in B5 medium，which were 2.314 mg?g-1 and 3.213 mg?g-1，respectively.1/4MS was the lowest，indicating that B5 was the best medium for root growth and production of Pterocephalus hookeri.

References:

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Last Update: 2019-10-08