|Table of Contents|

Study on Tissue Culture Technology of Opisthopappus taihangensis

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年06
Page:
83-88
Research Field:
Publishing date:

Info

Title:
Study on Tissue Culture Technology of Opisthopappus taihangensis
Author(s):
HAN ShuangMA LiPEI Dongli
(Department of Biological and Food,Shangqiu Normal University/Key Laboratory of Plant-Microbe interactions,Shangqiu,Henan 476000)
Keywords:
Opisthopappus taihangensiscallustissue culture
PACS:
-
DOI:
10.11937/bfyy.20182615
Abstract:
The leaves,petioles,stem tips and stem segments of Opisthopappus taihangensis were used as test materials,and different explants of Opisthopappus taihangensis were cultured with MS medium,and different callus were cultured to grow callus.The method of regenerating plants of the endangered Opisthopappus taihangensis was studied.The results showed that induction rate of callus induction from leaves and petioles of Opisthopappus taihangensis were 44.44% and 44.11%,respectively,which took longer time.Induction rate of callus induction from stem tip and stem segments were 48.44% and 68.75%,respectively and those took less time.Thus,we proved that stem segments were the most suitable materials for callus induction for Opisthopappus taihangensis.In order to effectively induce callus from stem segments,the combination of hormone concentration in callus of stem segments was studied,Group A,MS+6-BA 1.0 mg?L-1+NAA 0.5 mg?L-1+2,4-D 0.1 mg?L-1,Group B,MS+6-BA 1.0 mg?L-1+NAA 1.0 mg?L-1+2,4-D 0.1 mg?L-1,Group C,MS+6-BA 2.0 mg?L-1+NAA 0.5 mg?L-1+2,4-D 0.1 mg?L-1,Group D,MS+6-BA 2.0 mg?L-1+NAA 1.0 mg?L-1+2,4-D 0.1 mg?L-1.The test showed that the concentration of hormone in Group C was the most suitable for callus induction in the stem segment of Opisthopappus taihangensis.After 35 days,the callus was no longer increased and subcultured.After 45 days,the plants were transferred to rooting medium 1/2 MS+NAA 0.12 mg?L-1 for rooting culture.

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Last Update: 2019-04-18