|Table of Contents|

Optimization Extraction of Polyphenols and its Antioxidant Activity From Ophiopogon japonicus Flowers Using Response Surface Methodology

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年02
Page:
1-6
Research Field:
Publishing date:

Info

Title:
Optimization Extraction of Polyphenols and its Antioxidant Activity From Ophiopogon japonicus Flowers Using Response Surface Methodology
Author(s):
CAO Xiaoyan1; 2;  YANG Haitao1; 2
（1.Shaanxi Key Laboratory of Catalysis, Shaanxi University of Technology, Hanzhong,Shaanxi 723000; 2.School of Chemical & Environmental Sciences, Shaanxi University of Technology, Hanzhong,Shaanxi 723000)
Keywords:
Ophiopogon japonicus flowers;  ultrasonic-assisted extraction;  polyphenols;  response surface methodology;  antioxidant activity
PACS:
-
DOI:
10.11937/bfyy.20182247
Abstract:
With single factor and response surface method, this study investigated the optimal process parameters for ultrasonic-assisted extracting total polyphenols from Ophiopogon japonicus flowers, which was sampled from the Qinba Mountains. Meanwhile, the scavenging effects of polyphenols from different parts of Ophiopogon japonicus on superoxide anion radical(O2-?), hydroxyl free radical(?OH) and nitrite were also examined. The results showed that the optimum condition for ultrasonic-assisted extracting of polyphenols from Ophiopogon japonicus flowers were as follows: extraction temperature 57 ℃, ethanol concentration 63%, ultrasonic time 31 minutes, ratio of material to liquid 1:19 g·mL-1). Under this condition, the content of polyphenols from Ophiopogon japonicus flowers was 32.95±0.14 mg·g-1. The polyphenols extraction contents from Ophiopogon japonicus tuber roots, leaf and fibrous root were 24.64 ± 0.05 mg·g-1, 20.52 ± 0.04 mg·g-1and 17.42 ± 0.03 mg·g-1, respectively. Comparing with conventional antioxidant ascorbic acid, total polyphenols from different parts of Ophiopogon japonicas extraction could be better removing superoxide anion radical(O2-?), hydroxyl free radical(?OH) and nitrite, and the order of scavenging free radicals was as follows: Ophiopogon japonicus flowers>tuberous root>leaf>fibrous root. The polyphenols from Ophiopogon japonicus flowers had certain scavenging ability on O2-?, ·OH and NaNO2 with IC50 of 0.41 ± 0.01 mg·mL-1, 0.097 ± 0.005 mg·mL-1and 0.021 ± 0.005 mg·mL-1 respectively, and scavenging free radical IC50 of Vc at the same concentration for O2-?, ·OH and NaNO2 were 0.80 ± 0.01 mg·mL-1, 0.14 ± 0.01 mg·mL-1 and 0.012 ± 0.002 mg·mL-1, respectively. The study proved that polyphenols from different parts of Ophiopogon japonicus extraction had relatively good antioxidant activity.

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Last Update: 2018-11-12