|Table of Contents|

Breeding for New Seedless Grapevine Varieties With Disease-resistance Using Embryo Rescue

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2019年03
Page:
44-54
Research Field:
Publishing date:

Info

Title:
Breeding for New Seedless Grapevine Varieties With Disease-resistance Using Embryo Rescue
Author(s):
ZHAO Jiong1; 2; 3; LUO Qiangwei4; WANG Yuejin1; 2; 3
(1.College of Horticulture,Northwest Agriculture and Forestry University,Yangling,Shaanxi 712100;2.State Key Laboratory of Crop Stress Biology in Arid Areas,Yangling,Shaanxi 712100;3.Ministry of Agriculture Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest,Yangling,Shaanxi 712100;4.Institute of Grapes and Melons on the Research in Xinjiang,Shanshan,Xinjiang 838200)
Keywords:
Keywords:grapevine; seedlessness; embryo rescue; disease-resistance; marker-assisted selection
PACS:
-
DOI:
10.11937/bfyy.20182152
Abstract:
Abstract:European seedless cultivars were taken as female parent，and some cultivars with high disease resistance were taken as male parent，such as ‘Beichun’‘Zuoyouhong’ and ‘Tangwei’.Young berries were gathered before embryo abortion，and hybrid progenies were collected via embryo rescue in vitro under aseptic conditions.The effects of parental genotype，concentration of sucrose and GA3 in embryo germination medium on the efficiency of breeding seedless grape by embryo rescue were studied，in addition，molecular markers were applied in screening seedlessness or disease resistance of hybrid seedlings in order to provide reference for improving embryo rescue efficiency and screen the new materials of seedless and disease resistance grape preliminarily.The results showed that 232 strains were obtained and 227 of them were domesticated and transplanted；‘Centennial Seedless’ and ‘Kunxiang Seedless’ were more suitable for female parent，because their embryo rescue efficiency were better.In the medium for embryo germination，the optimal sugar concentration was 20% and the optimal GA3 concentration was 05 mol?L-1.Two molecular markers for seedless grapevines，such as SCF27-2000 and SCC8-1018 were selected for detecting 124 hybrid progenies.The result showed that 74 hybrid progenies were identified and the seedless rate of hybrid progenies was 597%.For resistance to powdery mildew，the molecular marker ScORN3-R was used for 65 hybrid progenies，but the specific brand 760 bp was not detected.

References:

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Last Update: 2019-03-14