Abstract

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Author(s):: ZHU Jinxia1; LI Miao1; ZHANG Yuanpei1; KONG Dejie1; ZHENG Guobao1; QU Jisong2; (1.Agricultural Biotechnology Center,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan,Ningxia 750002;2.Institute of Germplasm Resources,Ningxia Academy of Agriculture and Forestry Science,Yinchuan,Ningxia 750002)

Keywords:: microplate reader; colorimetric method; purple potato; total anthocyanin

Abstract:: Purple potato was taken as test material,the microplate reader as the instrument,established the rapid batch determination method,which could be used in the determination of the total anthocyanin content in purple potato.The results showed that the best experimental conditions for the detection of total anthocyanin content in purple potato by microplate reader,were the best suitable wavelength was 530 nm,a mixed solution(2∶1 volume ratio) of citric acid(solvent of 1.5%) and ethanol as the extraction solvent,the solid-liquid ratio was 1∶50 g·mL-1 and the ultrasound time was 40 minutes.Under these conditions,the extraction rate of total anthocyanin content in the purple potato was about 160.84 mg·(100g)-1.The method was sensitive,stable,and reproducible,and could be use to determine 96 samples at the same time in 2 minutes,but the results obtained by this method was slightly higher than those obtained by spectrophotometry.