Abstract

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Author(s):: HOU Hongmin1; 2; WANG Yaping1; 2; LIN Yanxiang1; 2; (1.College of Horticulture,Qingdao Agricultural University,Qingdao,Shandong 266109;2.Qingdao Key Laboratory of Genetic Development and Breeding in Horticultural Plants,Qingdao,Shandong 266109)

Abstract:: Chinese wild Vitis pseudoreticulata clone ‘Baihe-35-1’ was used as material,the DNA and cDNA sequences of VpSBP12 were isolated by using the primers designed based on the Grape Genome Database (http://www.genoscope.cns.fr) to analyze its sequence characteristics.The bioinformatics and gene expression analysis of VpSBP12 could help to elucidate the functional characterization of VpSBP12 in salt stress responses,and further provide a theoretical foundation for grape resistant breeding.The results indicated that the DNA sequence of VpSBP12 comprised 2 907 bp,which contained 3 exons and 2 intron.The VpSBP12 cDNA sequence included a 1 137 bp open reading frame (ORF) which encoded a polypeptide of 379 amino acids including a highly conserved SBP-domain bearing a nuclear localization signal (NLS).It was confirmed that they were targeted to the nucleus and possessed transcriptional activation activity by subcellular localization and trans-activation assay.The over-expression vector pCAMBIA2300-35S-VpSBP12 was constructed and transformed into Arabidopsis thaliana by floral dip methods.The VpSBP12 transgenic lines showed higher germination rate and longer roots than the wild type under the salt stress.These results indicated that over-expression of VpSBP12 in Arabidopsis could improve the toleration to salt stress.