Abstract

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Title:: Study on Tissue Culture and Rapid Propagation System of Cymbidium faberi Rolfe

Author(s):: FU Xiuqin; ZHOU Hailin; FANG Zhongming; HUANG Weiting; (Center of Applied Biotechnology，Wuhan University of Bioengineering，Wuhan，Hubei 430415)

Keywords:: Cymbidium faberi Rolfe; seed germination; rhizome; additives; proliferation; differentiation

Abstract:: In order to optimize tissue culture and rapid propagation system of Cymbidium faberi Rolfe，the seeds，rhizomes and aseptic seedlings of Cymbidium faberi Rolfe were used as materials to study the appropriate medium in the seed germination，the proliferation of rhizomes and the differentiation stage，and the matrix of seedling transplanting using tissue culture method.The results showed that the optimum medium for the seed germination of Cymbidium faberi Rolfe was 1/2MS+6-BA 0.5 mg·L-1+NAA 0.1 mg·L-1+activated carbon 1 g·L-1，the germination rate reached 80% after 90 days，and the suitable medium for the proliferation of Cymbidium faberi Rolfe was MS+6-BA 3 mg·L-1+NAA 1 mg·L-1+KT 1 mg·L-1+activated carbon 0.5 g·L-1+lysine 0.3 g·L-1，and the multiplication rate reached to 2.057 after 40 days；the best medium of bud differentiation from rhizome was MS+6-BA 8 mg·L-1+NAA 0.5 mg·L-1+sodium citrate 1.5 g·L-1+sorbitol 4 g·L-1，and the bud rate was 5.125 after 40 days；the best matrix combination of aseptic seedlings was king chestnut∶moss=2∶3，and the survival rate was 87.50% after 60 days.