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Title:: Breeding and Cultivation Formula of Flammulina velutipes of anti-Trichoderma harzianum

Author(s):: LIU Kun′ang; ZHANG Genwei; MA Hong; YIN Shuli; LIU Meng; LI Shusheng; (Institute of Biology，Hebei Academy of Sciences，Shijiazhuang,Hebei 050081)

Keywords:: Flammulina velutipes; Trichoderma viride; disease resistance; cultivation formula

Abstract:: The strain SH9 which was isolated from the commercially available white enoki mushroom tissue was used as parents.A new variety of white Flammulina velutipes JK501 was selected by the method of multi-spore hybridization and the ability of resistance to Trichoderma viride was tested，and then the enzyme activity index，mushroom traits and molecular identification were profomed.The results showed that the myceliume of strain JK501 was denser，and grew faster than SH9，and its activity against T.viride was increased by 3.56 times.The activity of laccase，carboxymethyl cellulase，hemicellulase and amylase and the content of 16 kinds of amino acids were significantly increased.The best cultivation formula for JK501 was No.6 medium,cotton seed peel 42%，bran 18%，walnut sawdust 33%，soybean meal powder 5%，gypsum 2%.Finally，it was proved that JK501 was a new strain different from parental SH9 by ITS and ISSR polymorphism.