Expression Analysis of MAPK Gene of Muskmelon
《北方园艺》[ISSN:1001-0009/CN:23-1247/S]
- Issue:
- 2018年24
- Page:
- 21-26
- Research Field:
- Publishing date:
Info
- Title:
- Expression Analysis of MAPK Gene of Muskmelon
- Author(s):
- XIN Dongdong1; LI Guirong1; GUO Weili1; ZHENG Qiuyun2; GENG Xinli2
- (1.School of Horticulture Landscape Architecture,Henan Institute of Science and Technology,Xinxiang,Henan 453003；2.Xinjiang Shanshan Grape Fruit Institute,Shanshan,Xinjiang 838200)
- Keywords:
- melon; melon powdery mildew; MAPK gene; expression analysis
- PACS:
- -
- DOI:
- 10.11937/bfyy.20181707
- Abstract:
- ‘Hami Jiageda’ and ‘Edisto47’ melon were used as experimental materials,by quantitative reverse-transcription polymerase chain reaction (qRT-PCR) method to study the expression of the MAPK genes,screening of melon powdery mildew resistance genes and analyses the expression of resistance genes,in order to provide references for regulatory function of the melon MAPK genes and the breeding of melon against powdery mildew.The results showed that the expression of MAPK9and MAPK21 [STBZ]was up-regulated after SA treatment for 9 hours of disease-resistant melon leaves,and the expression of MAPK21gene was the largest,the expression of MAPK21gene was up-regulated after SA treatment for 3 hours,24 hours and 72 hours of susceptible melon leaves.The expression levels of MAPK9 [STBZ]and MAPK21were significantly up-regulated after ETH treated the disease-resistant melon leaves for 6 hours.The expression of MAPK21gene was the greatest after ETH treated the susceptible melon leaves for 48 hours.The expression of MAPK9gene was significantly up-regulated after pumpkin powdery mildew spore treatment for 12 hours of disease-resistant melon leaves.The expression level of MAPK9 [STBZ]gene was significantly up-regulated after pumpkin powdery mildew spore treatment for 24 hours of susceptible melon leaves.To sum up,SA,ETH and pumpkin powdery mildew pathogen could induce the expression of this gene in melon leaves.Among them,both MAPK9 and MAPK21 were able to respond to SA induction after SA treatment for 9 hours of disease-resistant melon leaves and SA treatment for susceptible leaves for 3 hours,24 hours,72 hours,respectively.MAPK9was able to respond to the induction of in ETH and pumpkin powdery mildew spores.
Last Update: 2019-01-22