Abstract

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Title:: Genetic Diversity Analysis of Pomegranate Germplasm Resources From Zaozhuang by ISSR Markers

Author(s):: MA Li1; WANG Qingjun2; LUO Hua2; HAO Zhaoxiang2; ZHOU Yuliang1; ZHAO Lina2; (1.Life Science Department,Zaozhuang College,Zaozhuang,Shandong 277160;2.Zaozhuang Pomegranate Research Center/Yicheng District Fruit Tree Center in Zaozhuang,Zaozhuang,Shandong 277300)

Keywords:: Zaozhuang; pomegranate（Punica granatum L.）; genetic diversity; genetic relationships; ISSR

Abstract:: The 39 pomegranate cultivars from Zaozhuang were used as experimental materials in this study.The genetic relationships and genetic diversity of 39 pomegranate cultivars were analysed by ISSR markers in order to provided the foundation on the storage and breeding of the pomegranate resources at Zaozhuang.The results showed that a total of 168 loc were amplified from 9 ISSR primers with an average of 18.67 bands from a primer,of which 156 were polymorphic loci.The main indexes were,that percentage of polymorphic band (PPB)＝92.86%，the number of alleles (Na)=1.928 6，Effective number of alleles (Ne)=1.690 9,Nei′s gene diversity (He)=0.384 7 and Shannon′s information index (I)=0.557 3.The genetic similarities coefficient (GS) between cultivars ranged from 0.410 7 to 0.958 3,39 pomegranate cultivars were divided into 6 groups at GS 0.656 based on UPGMA method.The results of ISSR cluster did not accord with the classification results based on some morphological variations.