Abstract

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Title:: Analysis of Carmine Radish Genetic Relationship Based on ITS Sequences

Author(s):: LIANG Shan; YANG Xia; (College of Modern Agriculture and Biological Engineering,Yangtze Normal University，Chongqing 408100)

Keywords:: carmine radish; ITS sequence; genetic distance; genetic relationship

Abstract:: To detect the genetic relationship of carmine radish,carmine radish was taken as the material,the complete ITS1-5.8S rRNA-ITS2 sequence was amplified by PCR,and homology analysis and phylogenetic tree were constructed.The results showed the length of ITS1-5.8S rRNA-ITS2 sequences was 655 bp,the （G+C）content of ITS1 （220 bp）was 51.4%,5.8S rRNA（217 bp） was 53.4%，ITS2 （218 bp）was 55.1%.The homology analysis showed carmine radish,Taipei（Mei-Hwa）,Nanjing（Cd-03）,Taipei（Mei-Nong）,Nanjing（Yanghua）,Hangzhou（Baiyuchun）,and Ottawa（SW77） belonged to the same species with the highest homology （99%）.The phylogenetic tree showed the average genetic distance of the 20 samples was 0.134.Combined with morphological features indicated that carmine radish may be evolved from radish with white episperm and white heart.